Detailed information NTG0110134

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACAK28_RS14110
• Genome accession: NZ_AP031577
• Coordinates: 2965920..2967038 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain JUNP403
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2960920..2972038

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAK28_RS14100 (JUNP403_2831)	-	2964036..2964728 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACAK28_RS14105 (JUNP403_2832)	pilT	2964855..2965892 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACAK28_RS14110 (JUNP403_2833)	pilU	2965920..2967038 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACAK28_RS14115 (JUNP403_2834)	fur	2967108..2967545 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACAK28_RS14120 (JUNP403_2835)	-	2967657..2968055 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACAK28_RS14125 (JUNP403_2836)	-	2968110..2968433 (-)	324	WP_000442587.1	RnfH family protein	-
ACAK28_RS14130 (JUNP403_2837)	-	2968433..2969500 (-)	1068	WP_002019572.1	hypothetical protein	-
ACAK28_RS14135 (JUNP403_2838)	-	2969653..2970108 (-)	456	WP_000782976.1	bacteriohemerythrin	-
ACAK28_RS14140 (JUNP403_2839)	-	2970361..2971209 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=110134 ACAK28_RS14110 WP_000347039.1 2965920..2967038(+) (pilU) [Acinetobacter baumannii strain JUNP403]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=110134 ACAK28_RS14110 WP_000347039.1 2965920..2967038(+) (pilU) [Acinetobacter baumannii strain JUNP403]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395