Detailed information NTG0110087

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACAK35_RS14240
• Genome accession: NZ_AP031576
• Coordinates: 3009804..3010922 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain JUNP402
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3004804..3015922

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAK35_RS14230 (JUNP402_2847)	-	3007920..3008612 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACAK35_RS14235 (JUNP402_2848)	pilT	3008739..3009776 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
ACAK35_RS14240 (JUNP402_2849)	pilU	3009804..3010922 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACAK35_RS14245 (JUNP402_2850)	fur	3010992..3011429 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACAK35_RS14250 (JUNP402_2851)	-	3011541..3011939 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACAK35_RS14255 (JUNP402_2852)	-	3011994..3012317 (-)	324	WP_000442585.1	RnfH family protein	-
ACAK35_RS14260 (JUNP402_2853)	-	3012317..3013384 (-)	1068	WP_000092403.1	hypothetical protein	-
ACAK35_RS14265 (JUNP402_2854)	-	3013537..3013992 (-)	456	WP_000782976.1	bacteriohemerythrin	-
ACAK35_RS14270 (JUNP402_2855)	-	3014245..3015093 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=110087 ACAK35_RS14240 WP_000347036.1 3009804..3010922(+) (pilU) [Acinetobacter baumannii strain JUNP402]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=110087 ACAK35_RS14240 WP_000347036.1 3009804..3010922(+) (pilU) [Acinetobacter baumannii strain JUNP402]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392