Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACM66N_RS24350 Genome accession   NZ_CP182111
Coordinates   5095186..5096403 (-) Length   405 a.a.
NCBI ID   WP_416637920.1    Uniprot ID   -
Organism   Pseudomonas sp. OHS18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5090186..5101403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM66N_RS24325 (ACM66N_24325) - 5090242..5091012 (+) 771 WP_416637916.1 3-hydroxybutyrate dehydrogenase -
  ACM66N_RS24330 (ACM66N_24330) - 5091072..5093027 (+) 1956 WP_416637917.1 acetoacetate--CoA ligase -
  ACM66N_RS24335 (ACM66N_24335) - 5093173..5093889 (+) 717 WP_416637918.1 substrate-binding periplasmic protein -
  ACM66N_RS24340 (ACM66N_24340) - 5093957..5094475 (+) 519 WP_312230276.1 thioredoxin family protein -
  ACM66N_RS24345 (ACM66N_24345) - 5094472..5095083 (-) 612 WP_416637919.1 DJ-1/PfpI family protein -
  ACM66N_RS24350 (ACM66N_24350) pilU 5095186..5096403 (-) 1218 WP_416637920.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM66N_RS24355 (ACM66N_24355) - 5096547..5096825 (+) 279 WP_013791549.1 peptidylprolyl isomerase -
  ACM66N_RS24360 (ACM66N_24360) sseA 5096936..5097787 (+) 852 WP_416637921.1 3-mercaptopyruvate sulfurtransferase -
  ACM66N_RS24365 (ACM66N_24365) - 5098043..5098773 (-) 731 Protein_4792 ABC transporter ATP-binding protein -
  ACM66N_RS24370 (ACM66N_24370) - 5098770..5099594 (-) 825 WP_416637922.1 ABC transporter permease -
  ACM66N_RS24375 (ACM66N_24375) - 5099581..5100548 (-) 968 Protein_4794 aliphatic sulfonate ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44671.05 Da        Isoelectric Point: 6.0817

>NTDB_id=1100421 ACM66N_RS24350 WP_416637920.1 5095186..5096403(-) (pilU) [Pseudomonas sp. OHS18]
MDLHAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSSEPLKAGAVAEIAEGVMDGAQREEFERELEMNLAISLNGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVLLGTPTIRDLIKRNEFSEIKEIMEKSKNLGMQTFDQAL
IDLVNEGAIDEEEAVKNADSANNVRLKLKLYRETPAVPAPAAQPAAAVAEVRPAKPAEPADWGLELKLEDIEQDAPPEDP
GRHGI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1100421 ACM66N_RS24350 WP_416637920.1 5095186..5096403(-) (pilU) [Pseudomonas sp. OHS18]
ATGGATCTTCACGCAATGCTGAAAATACTCTCCAGCCAGGATGGCTCGGACCTGTACCTGTCGACCGGTGCGCCACCCTG
CGCCAAGTTCAACGGCGTGCTCAAGGCGCTGAGCAGCGAGCCGCTGAAGGCCGGCGCCGTGGCGGAAATCGCCGAAGGGG
TGATGGATGGCGCCCAGCGCGAAGAGTTCGAGCGCGAGCTGGAAATGAACCTGGCGATCTCCCTCAACGGCGTCGGGCGC
TTCCGCATCAATATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTCGACATTCCGCGCTTCGA
GGATCTGAAACTGCCCGAGGTGCTGCTCAAGACGGTGATGGAAAAACGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAGTCGACCTCGCTGGCCGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAC
CCGGTGGAATACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGCGTGGACACCCGCAGCTTCCACGC
CGCACTGAAGAATACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CTCTGGCGTTTGCCGATACCGGGCACCTGGCGATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATT
ATCAACTTCTTCCCCGAAGACCGCCGCCCGCAATTGCTCAATGACCTGGGCAACAACCTCAAGGCGTTCGTTTCCCAGCG
CCTGGTCAAGACGGTCGATGGCAAGCGCCGCGCCGCAGTCGAGGTGCTGCTCGGCACGCCGACCATCCGTGACCTGATCA
AGCGCAACGAGTTTTCCGAAATCAAGGAGATCATGGAAAAGTCCAAGAACCTCGGCATGCAGACCTTCGACCAGGCGCTG
ATCGACCTGGTCAACGAAGGCGCCATCGACGAGGAGGAGGCGGTCAAGAACGCCGACTCGGCCAATAACGTGCGCCTCAA
GCTCAAGCTCTACCGTGAGACGCCAGCCGTTCCGGCCCCCGCAGCCCAGCCAGCGGCTGCGGTGGCCGAGGTTCGCCCGG
CCAAGCCGGCCGAGCCCGCAGACTGGGGGCTGGAGCTGAAGCTCGAAGATATCGAGCAAGACGCGCCACCCGAGGATCCG
GGCCGCCACGGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.183

87.654

0.501
  pilU Acinetobacter baylyi ADP1

52.247

87.901

0.459
  pilU Vibrio cholerae strain A1552

51.558

87.16

0.449
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.108

94.074

0.368

Multiple sequence alignment