Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   HW00_RS03140 Genome accession   NZ_CP008867
Coordinates   671398..672456 (-) Length   352 a.a.
NCBI ID   WP_003086123.1    Uniprot ID   Q02IC9
Organism   Pseudomonas aeruginosa strain T52373     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 666398..677456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW00_RS03110 (HW00_03115) pal 666839..667345 (-) 507 WP_003111417.1 peptidoglycan-associated lipoprotein Pal -
  HW00_RS03115 (HW00_03120) tolB 667398..668696 (-) 1299 WP_003086128.1 Tol-Pal system beta propeller repeat protein TolB -
  HW00_RS03120 (HW00_03125) tolA 668693..669736 (-) 1044 WP_003117096.1 cell envelope integrity protein TolA -
  HW00_RS03125 (HW00_03130) tolR 669739..670179 (-) 441 WP_003086126.1 protein TolR -
  HW00_RS03130 (HW00_03135) tolQ 670202..670897 (-) 696 WP_003086125.1 protein TolQ -
  HW00_RS03135 (HW00_03140) ybgC 670899..671345 (-) 447 WP_003086124.1 tol-pal system-associated acyl-CoA thioesterase -
  HW00_RS03140 (HW00_03145) ruvB 671398..672456 (-) 1059 WP_003086123.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  HW00_RS03145 (HW00_03150) ruvA 672467..673072 (-) 606 WP_003086122.1 Holliday junction branch migration protein RuvA -
  HW00_RS03150 (HW00_03155) ruvC 673089..673613 (-) 525 WP_003112575.1 crossover junction endodeoxyribonuclease RuvC -
  HW00_RS03155 (HW00_03160) - 673698..674444 (-) 747 WP_003086107.1 YebC/PmpR family DNA-binding transcriptional regulator -
  HW00_RS03160 (HW00_03165) aspS 674511..676286 (-) 1776 WP_003123218.1 aspartate--tRNA ligase -
  HW00_RS03165 (HW00_03170) - 676726..677196 (+) 471 WP_003086103.1 Dps family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38932.67 Da        Isoelectric Point: 5.1792

>NTDB_id=110004 HW00_RS03140 WP_003086123.1 671398..672456(-) (ruvB) [Pseudomonas aeruginosa strain T52373]
MIEPDRLISAVSGRERDEQLDRAIRPLKLADYIGQPSVREQMELFIHAARGRQEALDHTLIFGPPGLGKTTLANIIAQEM
GVSIKSTSGPVLERPGDLAALLTNLEAGDVLFVDEIHRLSPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLVG
ATTRAGMLTNPLRDRFGIVQRLEFYNVEDLATIVSRSAGILGLEIEPQGAAEIAKRARGTPRIANRLLRRVRDFAEVRGQ
GDITRVIADKALNLLDVDERGFDHLDRRLLLTMIDKFDGGPVGIDNLAAALSEERHTIEDVLEPYLIQQGYIMRTPRGRV
VTRHAYLHFGLNIPKRLGPGVTTDLFTSEDGN

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=110004 HW00_RS03140 WP_003086123.1 671398..672456(-) (ruvB) [Pseudomonas aeruginosa strain T52373]
ATGATCGAGCCTGATCGCCTCATATCCGCCGTTTCCGGTCGCGAACGCGATGAGCAGCTGGACCGCGCCATTCGCCCGCT
GAAGCTGGCCGACTACATCGGCCAGCCCAGCGTGCGCGAGCAGATGGAGCTGTTCATCCATGCCGCGCGCGGCCGCCAGG
AGGCGCTCGACCATACGCTGATCTTCGGTCCGCCGGGGCTGGGCAAGACGACCCTGGCGAATATCATCGCCCAGGAAATG
GGCGTTTCCATCAAGAGTACTTCCGGGCCGGTGCTCGAGCGTCCCGGCGATCTCGCCGCATTGCTGACCAACCTGGAGGC
CGGCGACGTCCTGTTCGTCGACGAGATCCACCGTCTTTCGCCCATCGTCGAAGAAGTGCTGTATCCGGCCATGGAAGATT
TCCAGCTGGACATCATGATCGGCGAAGGGCCCGCGGCCCGCTCCATCAAGCTGGACCTGCCGCCGTTCACCCTGGTCGGC
GCGACCACCCGTGCCGGCATGCTGACCAATCCACTGCGCGATCGCTTCGGTATCGTCCAGCGCCTGGAGTTCTACAACGT
CGAGGACCTGGCGACCATCGTCAGTCGGTCGGCCGGCATTCTCGGCCTGGAGATCGAGCCGCAGGGCGCGGCGGAAATCG
CCAAGCGCGCCCGCGGTACGCCGCGAATCGCCAATCGCCTGCTGCGCCGGGTACGCGACTTCGCCGAGGTCCGGGGGCAG
GGCGACATTACCCGGGTGATCGCCGACAAGGCATTGAACCTGCTCGATGTCGACGAGCGTGGCTTCGACCACCTCGACCG
TCGTCTGTTGCTGACCATGATCGACAAGTTCGATGGCGGGCCTGTAGGAATCGACAACTTGGCTGCGGCGTTGAGCGAGG
AGCGGCACACGATCGAAGATGTACTGGAGCCCTATCTGATCCAGCAGGGCTACATCATGCGGACACCGCGGGGGCGCGTG
GTAACACGCCATGCGTACCTGCACTTTGGCCTGAATATTCCGAAAAGGTTGGGGCCGGGGGTGACGACGGATCTGTTCAC
GTCTGAAGATGGTAATTAA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-329)

RuvB AAA lid domain (AAA_lid_4)

(185-258)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(24-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q02IC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.027

94.034

0.574
  ruvB Streptococcus pneumoniae TIGR4

59.062

90.909

0.537
  ruvB Streptococcus pneumoniae R6

58.75

90.909

0.534
  ruvB Streptococcus pneumoniae D39

58.75

90.909

0.534
  ruvB Synechocystis sp. PCC 6803

57.143

91.477

0.523
  ruvB Helicobacter pylori 26695

55.449

88.636

0.491

Multiple sequence alignment