Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACK2OW_RS08760 Genome accession   NZ_CP180575
Coordinates   1612441..1613832 (-) Length   463 a.a.
NCBI ID   WP_014481052.1    Uniprot ID   -
Organism   Bacillus subtilis strain K3C     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1607441..1618832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2OW_RS08725 (ACK2OW_08725) flgL 1607612..1608508 (-) 897 WP_416042642.1 flagellar hook-associated protein FlgL -
  ACK2OW_RS08730 (ACK2OW_08730) flgK 1608519..1610042 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  ACK2OW_RS08735 (ACK2OW_08735) flgN 1610061..1610543 (-) 483 WP_014481048.1 flagellar protein FlgN -
  ACK2OW_RS08740 (ACK2OW_08740) flgM 1610559..1610825 (-) 267 WP_015384783.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACK2OW_RS08745 (ACK2OW_08745) yvyF 1610906..1611325 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  ACK2OW_RS08750 (ACK2OW_08750) comFC 1611399..1612121 (-) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  ACK2OW_RS08755 (ACK2OW_08755) comFB 1612085..1612381 (-) 297 WP_003227989.1 late competence protein ComFB -
  ACK2OW_RS08760 (ACK2OW_08760) comFA 1612441..1613832 (-) 1392 WP_014481052.1 ATP-dependent helicase ComFA Machinery gene
  ACK2OW_RS08765 (ACK2OW_08765) fakBA 1613938..1614783 (-) 846 WP_014481053.1 DegV family protein -
  ACK2OW_RS08770 (ACK2OW_08770) degU 1614881..1615570 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  ACK2OW_RS08775 (ACK2OW_08775) degS 1615653..1616810 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  ACK2OW_RS08780 (ACK2OW_08780) yvyE 1617027..1617680 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52543.49 Da        Isoelectric Point: 9.7976

>NTDB_id=1096771 ACK2OW_RS08760 WP_014481052.1 1612441..1613832(-) (comFA) [Bacillus subtilis strain K3C]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=1096771 ACK2OW_RS08760 WP_014481052.1 1612441..1613832(-) (comFA) [Bacillus subtilis strain K3C]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGGGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCCGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.056

100

0.981

Multiple sequence alignment