Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACMZ68_RS16545 Genome accession   NZ_CP180522
Coordinates   3464713..3465465 (-) Length   250 a.a.
NCBI ID   WP_206216692.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AP5091     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3459713..3470465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMZ68_RS16525 (ACMZ68_16525) aroB 3460291..3461376 (-) 1086 WP_336030421.1 3-dehydroquinate synthase -
  ACMZ68_RS16530 (ACMZ68_16530) aroK 3461405..3461974 (-) 570 WP_002116987.1 shikimate kinase AroK -
  ACMZ68_RS16535 (ACMZ68_16535) pilQ 3461986..3464112 (-) 2127 WP_069120196.1 type IV pilus secretin PilQ Machinery gene
  ACMZ68_RS16540 (ACMZ68_16540) pilP 3464175..3464702 (-) 528 WP_069120199.1 pilus assembly protein PilP Machinery gene
  ACMZ68_RS16545 (ACMZ68_16545) pilO 3464713..3465465 (-) 753 WP_206216692.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACMZ68_RS16550 (ACMZ68_16550) pilN 3465462..3466103 (-) 642 WP_032004890.1 PilN domain-containing protein Machinery gene
  ACMZ68_RS16555 (ACMZ68_16555) pilM 3466103..3467161 (-) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  ACMZ68_RS16560 (ACMZ68_16560) ponA 3467323..3469884 (+) 2562 WP_032036609.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28301.20 Da        Isoelectric Point: 4.8782

>NTDB_id=1096206 ACMZ68_RS16545 WP_206216692.1 3464713..3465465(-) (pilO) [Acinetobacter pittii strain AP5091]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGKSDIPVLNYFIKAKTYRYMGAVENPEHQEKNASASSVAP
DSSTANTQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=1096206 ACMZ68_RS16545 WP_206216692.1 3464713..3465465(-) (pilO) [Acinetobacter pittii strain AP5091]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTACTGGCTTTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGCGTGAATTCTGGTCTGAAGTTTAAAAATATTCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTTTATCAAAG
CAAAAACTTATCGCTATATGGGTGCTGTAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAATACACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.2

100

0.832
  comO Acinetobacter baylyi ADP1

69.583

96

0.668

Multiple sequence alignment