Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMYQ0_RS06820 Genome accession   NZ_CP180477
Coordinates   1424584..1425816 (+) Length   410 a.a.
NCBI ID   WP_415755301.1    Uniprot ID   -
Organism   Pseudomonas leptonychotis strain CCM 8849     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1419584..1430816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMYQ0_RS06800 - 1420064..1421650 (-) 1587 WP_136664701.1 ABC-F family ATPase -
  ACMYQ0_RS06805 sph 1422137..1423651 (-) 1515 WP_415755300.1 sphingomyelin phosphodiesterase -
  ACMYQ0_RS06810 - 1423785..1424153 (-) 369 WP_136665153.1 MmcQ/YjbR family DNA-binding protein -
  ACMYQ0_RS06815 - 1424162..1424440 (-) 279 WP_090253882.1 peptidylprolyl isomerase -
  ACMYQ0_RS06820 pilU 1424584..1425816 (+) 1233 WP_415755301.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMYQ0_RS06825 - 1425823..1427778 (-) 1956 WP_415755302.1 acetoacetate--CoA ligase -
  ACMYQ0_RS06830 - 1428006..1428908 (-) 903 WP_415755303.1 hydroxymethylglutaryl-CoA lyase -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44915.25 Da        Isoelectric Point: 5.7235

>NTDB_id=1095974 ACMYQ0_RS06820 WP_415755301.1 1424584..1425816(+) (pilU) [Pseudomonas leptonychotis strain CCM 8849]
MDLNAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSAEPLKAGEVADIADAVMDVAQREEFERELEMNLAISIAGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLKLPHVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHKHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTIDGKRRAAVEVLLGTPTIRDLIKRNEFSEIKEIMEKSKNLGMQTFDQAL
IDLVNDGSIDEDEAVKNADSANNVRLKLKLYRDNPAAPVQAAAASAPAPAAAEARSTTPAASAGEWGLELTLEDIEEEPL
PPEDPGRKGI

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=1095974 ACMYQ0_RS06820 WP_415755301.1 1424584..1425816(+) (pilU) [Pseudomonas leptonychotis strain CCM 8849]
ATGGATCTCAACGCCATGCTGAAAATCCTGTCCAGTCAGGACGGGTCTGATCTTTACCTTTCGACTGGAGCACCGCCGTG
CGCCAAGTTCAATGGCGTGCTCAAGGCGTTGAGTGCTGAACCGCTGAAAGCCGGCGAGGTGGCGGATATTGCAGACGCGG
TGATGGATGTGGCGCAGCGTGAAGAGTTCGAGCGCGAGTTGGAGATGAACCTGGCTATTTCCATTGCTGGCGTCGGGCGC
TTTCGTATCAATATCTTCAAGCAGCGCAACGAAGTGTCGATTGTGGCGCGCAATATCAAGTTGGACATCCCCAAGTTTGA
AGACCTTAAACTCCCGCATGTGCTGCTCAAGACGGTGATGGAAAAGCGCGGCCTGGTGCTGTTCGTTGGCGGCACGGGCT
CGGGTAAATCGACCTCGCTGGCCGCGTTGATTGATTACCGTAACCGCAACAGCGGTGGTCATATCATCACCATCGAAGAC
CCGGTGGAGTATGTACACAAGCACAAAAAGTCGATCATCAACCAACGCGAAGTCGGCGTAGATACCCGCAGTTTTCACGC
GGCACTGAAGAACACCCTGCGCCAGGCCCCAGACGTGATCCTGATCGGCGAAATTCGCGACCGCGAAACCATGGAACATG
CCCTGGCCTTTGCCGACACCGGCCACCTGGCAATTTCCACGCTACACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGACCGACGCCCGCAACTGCTTAACGACCTGGGTAACAACCTCAAAGCATTCGTCTCGCAGCG
CCTGGTGAAAACCATCGACGGTAAACGCCGCGCGGCGGTTGAAGTACTGCTGGGCACGCCGACCATTCGCGACCTGATCA
AACGCAACGAGTTTTCCGAGATCAAGGAGATCATGGAGAAATCGAAGAACCTCGGCATGCAAACCTTCGATCAAGCGCTG
ATCGACTTGGTCAACGATGGCTCGATTGACGAAGACGAAGCGGTGAAAAATGCCGACTCGGCAAACAACGTGCGATTGAA
GCTCAAACTGTATCGCGACAATCCAGCTGCGCCGGTGCAGGCCGCTGCAGCCTCGGCACCCGCTCCAGCGGCTGCCGAGG
CACGGTCGACAACCCCAGCCGCTTCAGCGGGCGAGTGGGGGCTGGAGCTGACGCTGGAGGATATCGAAGAGGAGCCACTG
CCGCCTGAAGACCCAGGGCGCAAAGGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.41

85.61

0.483
  pilU Acinetobacter baylyi ADP1

52.557

85.854

0.451
  pilU Vibrio cholerae strain A1552

52.125

86.098

0.449
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.481

96.341

0.371

Multiple sequence alignment