Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   ACLBKW_RS11425 Genome accession   NZ_CP180232
Coordinates   2238999..2239292 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus altitudinis strain Ba     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2233999..2244292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLBKW_RS11375 (ACLBKW_11375) sinR 2234169..2234504 (+) 336 WP_017357978.1 transcriptional regulator SinR Regulator
  ACLBKW_RS11380 (ACLBKW_11380) tasA 2234572..2235372 (-) 801 WP_008342195.1 biofilm matrix protein TasA -
  ACLBKW_RS11385 (ACLBKW_11385) sipW 2235431..2236003 (-) 573 WP_017357979.1 signal peptidase I SipW -
  ACLBKW_RS11390 (ACLBKW_11390) tapA 2236053..2236580 (-) 528 WP_304717077.1 amyloid fiber anchoring/assembly protein TapA -
  ACLBKW_RS11395 (ACLBKW_11395) - 2236833..2237159 (+) 327 WP_017367590.1 DUF3889 domain-containing protein -
  ACLBKW_RS11400 (ACLBKW_11400) - 2237194..2237382 (-) 189 WP_414834365.1 YqzE family protein -
  ACLBKW_RS11405 (ACLBKW_11405) - 2237443..2237826 (-) 384 WP_039964023.1 competence type IV pilus minor pilin ComGG -
  ACLBKW_RS11410 (ACLBKW_11410) comGF 2237823..2238320 (-) 498 WP_250026311.1 competence type IV pilus minor pilin ComGF -
  ACLBKW_RS11415 (ACLBKW_11415) comGE 2238262..2238576 (-) 315 WP_148780034.1 competence type IV pilus minor pilin ComGE -
  ACLBKW_RS11420 (ACLBKW_11420) comGD 2238560..2239006 (-) 447 WP_250026309.1 competence type IV pilus minor pilin ComGD -
  ACLBKW_RS11425 (ACLBKW_11425) comGC 2238999..2239292 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLBKW_RS11430 (ACLBKW_11430) comGB 2239309..2240349 (-) 1041 WP_250026308.1 competence type IV pilus assembly protein ComGB -
  ACLBKW_RS11435 (ACLBKW_11435) comGA 2240330..2241400 (-) 1071 WP_019743634.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLBKW_RS11440 (ACLBKW_11440) - 2241857..2242729 (-) 873 WP_046527023.1 STAS domain-containing protein -
  ACLBKW_RS11450 (ACLBKW_11450) - 2242955..2243335 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACLBKW_RS11455 (ACLBKW_11455) - 2243548..2243790 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=1094683 ACLBKW_RS11425 WP_008342186.1 2238999..2239292(-) (comGC) [Bacillus altitudinis strain Ba]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=1094683 ACLBKW_RS11425 WP_008342186.1 2238999..2239292(-) (comGC) [Bacillus altitudinis strain Ba]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTCATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCAAATGGAAAAGCAATTTTCATTCAAAACGGCGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment