Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACMHYC_RS01980 Genome accession   NZ_CP180027
Coordinates   418091..418810 (+) Length   239 a.a.
NCBI ID   WP_009506583.1    Uniprot ID   K9C0F4
Organism   Acinetobacter courvalinii isolate 2024CK-01798     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413091..423810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMHYC_RS01965 (ACMHYC_01965) ponA 413622..416216 (-) 2595 WP_081401585.1 penicillin-binding protein PBP1a -
  ACMHYC_RS01970 (ACMHYC_01970) comM 416380..417438 (+) 1059 WP_005227665.1 pilus assembly protein PilM Machinery gene
  ACMHYC_RS01975 (ACMHYC_01975) pilN 417438..418094 (+) 657 WP_005227663.1 PilN domain-containing protein Machinery gene
  ACMHYC_RS01980 (ACMHYC_01980) pilO 418091..418810 (+) 720 WP_009506583.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACMHYC_RS01985 (ACMHYC_01985) pilP 418814..419341 (+) 528 WP_005227658.1 pilus assembly protein PilP Machinery gene
  ACMHYC_RS01990 (ACMHYC_01990) pilQ 419403..421535 (+) 2133 WP_171067683.1 type IV pilus secretin PilQ Machinery gene
  ACMHYC_RS01995 (ACMHYC_01995) aroK 421546..422115 (+) 570 WP_104021896.1 shikimate kinase AroK -
  ACMHYC_RS02000 (ACMHYC_02000) aroB 422135..423217 (+) 1083 WP_414659598.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 239 a.a.        Molecular weight: 26919.42 Da        Isoelectric Point: 4.6537

>NTDB_id=1094076 ACMHYC_RS01980 WP_009506583.1 418091..418810(+) (pilO) [Acinetobacter courvalinii isolate 2024CK-01798]
MNQDELNQDSVAVKKKKMTVEQFFQQFNTLDMNNYGSWPLSVKITCWIFVFLAVLALGYFVVIKSQLEAIENAQAQEQSL
LNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLENEVKQEFFIEQPISI
DATGDYHAFGAFVTGIAALPRIVTLHDFTVEAKEDTQKKSDIPVVNYTVKAKTYRYVGADDQANAAAAATNNPPAAGGK

Nucleotide


Download         Length: 720 bp        

>NTDB_id=1094076 ACMHYC_RS01980 WP_009506583.1 418091..418810(+) (pilO) [Acinetobacter courvalinii isolate 2024CK-01798]
ATGAACCAAGATGAATTGAATCAAGACAGCGTTGCGGTCAAGAAAAAGAAAATGACGGTCGAGCAGTTCTTCCAGCAATT
TAATACGCTGGATATGAACAATTACGGCAGTTGGCCTTTATCTGTCAAAATTACCTGCTGGATTTTTGTGTTTCTAGCCG
TGCTGGCGCTGGGTTATTTTGTGGTGATTAAATCTCAGCTTGAAGCGATTGAAAATGCTCAGGCCCAGGAACAGAGCTTG
CTCAACGAGTTTCGTGAAAAAGATTCCAAGCTGCGTAACTTGCAGCAATATCAAGCGCAACTACAAGAAATGGAAGCGAA
TTTTAATCAACAATTAGAGCAACTGCCAAAAGAGACAGAAATTCCAAGTCTGGTTGAAGACATTAACCTGACCGGAGTGA
ATTCAGGTCTGAAATTTAAAAATATCCGTCTGGAAAATGAAGTTAAGCAAGAGTTCTTTATTGAGCAACCGATCAGTATC
GATGCGACAGGTGACTACCATGCCTTTGGTGCATTTGTTACCGGTATTGCGGCTTTGCCTCGTATTGTTACCTTGCATGA
CTTTACCGTAGAAGCAAAAGAAGATACCCAGAAAAAATCGGATATTCCGGTGGTGAATTATACCGTGAAAGCCAAAACCT
ACCGCTATGTAGGTGCGGATGATCAGGCCAATGCCGCTGCGGCAGCGACAAATAATCCACCTGCTGCAGGAGGAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(70-217)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9C0F4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

75.833

100

0.762
  comO Acinetobacter baylyi ADP1

74.37

99.582

0.741

Multiple sequence alignment