Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACMGRF_RS14440 Genome accession   NZ_CP179923
Coordinates   3132476..3133534 (-) Length   352 a.a.
NCBI ID   WP_014038321.1    Uniprot ID   A0AAP7J1E4
Organism   Stenotrophomonas sp. EMP41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3127476..3138534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGRF_RS14420 (ACMGRF_14420) - 3128426..3130396 (-) 1971 WP_049438328.1 type IV pilus secretin PilQ -
  ACMGRF_RS14425 (ACMGRF_14425) - 3130418..3130951 (-) 534 WP_005418914.1 pilus assembly protein PilP -
  ACMGRF_RS14430 (ACMGRF_14430) - 3130951..3131607 (-) 657 WP_049411380.1 type 4a pilus biogenesis protein PilO -
  ACMGRF_RS14435 (ACMGRF_14435) - 3131604..3132476 (-) 873 WP_100478696.1 PilN domain-containing protein -
  ACMGRF_RS14440 (ACMGRF_14440) pilM 3132476..3133534 (-) 1059 WP_014038321.1 pilus assembly protein PilM Machinery gene
  ACMGRF_RS14445 (ACMGRF_14445) - 3133754..3136177 (+) 2424 WP_414485341.1 penicillin-binding protein 1A -
  ACMGRF_RS14450 (ACMGRF_14450) - 3136388..3137032 (+) 645 WP_005418931.1 hypothetical protein -
  ACMGRF_RS14455 (ACMGRF_14455) - 3137148..3137894 (-) 747 WP_049411390.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37741.35 Da        Isoelectric Point: 4.4006

>NTDB_id=1093868 ACMGRF_RS14440 WP_014038321.1 3132476..3133534(-) (pilM) [Stenotrophomonas sp. EMP41]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPADLDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGSIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1093868 ACMGRF_RS14440 WP_014038321.1 3132476..3133534(-) (pilM) [Stenotrophomonas sp. EMP41]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTTGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGCGGCAATCGTTTCCGCGTGGAACATTACGCTGTGGAACCTCTTCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TTGAAGTGGAGGCCGTCGGTGAGGCCATCCGCCGGGCGATGAACCGTTCAGGCAGCAAGGCCAAGTTGGCCGCTGCCGCC
GTCGCGGGGTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGATCTCGACGAAAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTATCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGTCGATCCCGAACA
ACCCGGAGATGGTCCAGGTCCTGCTGGCCGCGTCGCGTTCGGAGAATGTCGAGCTGCGCCAGTCCGCACTGGAGCTCGGT
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTTGCGGTCGAGAACGCCTACGCCCTGGTCGCCAGCGAGCTGCCGGT
GTCCATCGACGGCGTGGTTGCGCTGGTCGACATCGGCGCCACCATGACCACCCTCAATGTCCTGCGCGGCGGCCGTAGCC
TTTACAGCCGCGAGCAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGCTATGGCCTGAGCTACGAGGAA
GCCGGGCTGGCCAAGCGCCAGGGTGGCCTGCCGGAAAGCTACGAAATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTATGCGGGCAGCGAATTCAACCGCGTCGATCACATCGTGCTGGCCGGCG
GCTGCGCCGTGCTCGGTGGCCTGCCGGAGATGGTCGAGGAGCAGCTGGGCGTGCCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTGAACGCGCATGCGTTGGCCCAGGATGCCCCCGCGCTGATGATCGCGACCGGTCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.42

100

0.514
  comM Acinetobacter nosocomialis M2

51.42

100

0.514
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

47.863

99.716

0.477

Multiple sequence alignment