Detailed information
Bioinformatically predicted
Overview
| Name | pilU | Type | Machinery gene |
| Locus tag | ACK8DA_RS04360 | Genome accession | NZ_CP179869 |
| Coordinates | 919027..920145 (-) | Length | 372 a.a. |
| NCBI ID | WP_000347040.1 | Uniprot ID | A0A809JLP7 |
| Organism | Acinetobacter baumannii strain MRSN122172 | ||
| Function | mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 882868..948561 | 919027..920145 | within | 0 |
Gene organization within MGE regions
Location: 882868..948561
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACK8DA_RS04180 (ACK8DA_04180) | - | 883269..883829 (-) | 561 | WP_001048354.1 | hypothetical protein | - |
| ACK8DA_RS04185 (ACK8DA_04185) | - | 883920..884780 (-) | 861 | WP_046693151.1 | pirin family protein | - |
| ACK8DA_RS04190 (ACK8DA_04190) | hemJ | 884951..885403 (-) | 453 | WP_000339889.1 | protoporphyrinogen oxidase HemJ | - |
| ACK8DA_RS04195 (ACK8DA_04195) | - | 885422..886651 (-) | 1230 | WP_004748109.1 | beta-ketoacyl-ACP synthase II | - |
| ACK8DA_RS04200 (ACK8DA_04200) | - | 886948..888273 (+) | 1326 | WP_004748107.1 | EcsC family protein | - |
| ACK8DA_RS04205 (ACK8DA_04205) | rpsL | 888474..888848 (+) | 375 | WP_000246374.1 | 30S ribosomal protein S12 | - |
| ACK8DA_RS04210 (ACK8DA_04210) | rpsG | 889008..889478 (+) | 471 | WP_001138055.1 | 30S ribosomal protein S7 | - |
| ACK8DA_RS04215 (ACK8DA_04215) | fusA | 889658..891796 (+) | 2139 | WP_004748104.1 | elongation factor G | - |
| ACK8DA_RS04220 (ACK8DA_04220) | tuf | 891891..893081 (+) | 1191 | WP_001029610.1 | elongation factor Tu | - |
| ACK8DA_RS04225 (ACK8DA_04225) | - | 893285..894166 (+) | 882 | WP_004748103.1 | metal-dependent hydrolase | - |
| ACK8DA_RS04230 (ACK8DA_04230) | - | 894403..895278 (+) | 876 | WP_000992291.1 | metal-dependent hydrolase | - |
| ACK8DA_RS04235 (ACK8DA_04235) | rimI | 895388..895843 (+) | 456 | WP_000621543.1 | ribosomal protein S18-alanine N-acetyltransferase | - |
| ACK8DA_RS04240 (ACK8DA_04240) | - | 895888..896703 (-) | 816 | WP_000844343.1 | arginyltransferase | - |
| ACK8DA_RS04245 (ACK8DA_04245) | aat | 896722..897453 (-) | 732 | WP_000854785.1 | leucyl/phenylalanyl-tRNA--protein transferase | - |
| ACK8DA_RS04250 (ACK8DA_04250) | trxB | 897573..898520 (-) | 948 | WP_001276144.1 | thioredoxin-disulfide reductase | - |
| ACK8DA_RS04255 (ACK8DA_04255) | - | 898790..901822 (+) | 3033 | WP_004748102.1 | DNA translocase FtsK | - |
| ACK8DA_RS04260 (ACK8DA_04260) | rhtC | 901971..902591 (+) | 621 | WP_004748101.1 | threonine export protein RhtC | - |
| ACK8DA_RS04265 (ACK8DA_04265) | - | 902647..903186 (-) | 540 | WP_001067701.1 | hypothetical protein | - |
| ACK8DA_RS04270 (ACK8DA_04270) | - | 903334..903621 (-) | 288 | WP_001218560.1 | PA4642 family protein | - |
| ACK8DA_RS04275 (ACK8DA_04275) | minE | 903705..903977 (-) | 273 | WP_000896934.1 | cell division topological specificity factor MinE | - |
| ACK8DA_RS04280 (ACK8DA_04280) | minD | 903980..904792 (-) | 813 | WP_001074362.1 | septum site-determining protein MinD | - |
| ACK8DA_RS04285 (ACK8DA_04285) | minC | 904863..905585 (-) | 723 | WP_000763678.1 | septum site-determining protein MinC | - |
| ACK8DA_RS04290 (ACK8DA_04290) | - | 905707..906753 (-) | 1047 | WP_001181640.1 | hypothetical protein | - |
| ACK8DA_RS04295 (ACK8DA_04295) | - | 906769..907695 (-) | 927 | WP_000100973.1 | acyltransferase | - |
| ACK8DA_RS04300 (ACK8DA_04300) | - | 907940..908593 (+) | 654 | WP_001202415.1 | OmpA family protein | - |
| ACK8DA_RS04305 (ACK8DA_04305) | rep | 908787..910826 (+) | 2040 | WP_142103771.1 | DNA helicase Rep | - |
| ACK8DA_RS04310 (ACK8DA_04310) | dut | 910851..911303 (+) | 453 | WP_000868158.1 | dUTP diphosphatase | - |
| ACK8DA_RS04315 (ACK8DA_04315) | - | 911503..912921 (+) | 1419 | WP_001102848.1 | phosphomannomutase/phosphoglucomutase | - |
| ACK8DA_RS04320 (ACK8DA_04320) | argB | 912936..913844 (+) | 909 | WP_001135419.1 | acetylglutamate kinase | - |
| ACK8DA_RS04325 (ACK8DA_04325) | - | 913965..914747 (+) | 783 | WP_000890282.1 | GNAT family N-acetyltransferase | - |
| ACK8DA_RS04330 (ACK8DA_04330) | - | 914856..915704 (+) | 849 | WP_000336554.1 | class II glutamine amidotransferase | - |
| ACK8DA_RS04335 (ACK8DA_04335) | - | 915957..916412 (+) | 456 | WP_000782976.1 | bacteriohemerythrin | - |
| ACK8DA_RS04340 (ACK8DA_04340) | - | 916565..917632 (+) | 1068 | WP_046693192.1 | membrane protein | - |
| ACK8DA_RS04345 (ACK8DA_04345) | - | 917632..917955 (+) | 324 | WP_000442590.1 | RnfH family protein | - |
| ACK8DA_RS04350 (ACK8DA_04350) | - | 918010..918408 (-) | 399 | WP_001170994.1 | outer membrane protein assembly factor BamE | - |
| ACK8DA_RS04355 (ACK8DA_04355) | fur | 918520..918957 (+) | 438 | WP_001122847.1 | ferric iron uptake transcriptional regulator | - |
| ACK8DA_RS04360 (ACK8DA_04360) | pilU | 919027..920145 (-) | 1119 | WP_000347040.1 | PilT/PilU family type 4a pilus ATPase | Machinery gene |
| ACK8DA_RS04365 (ACK8DA_04365) | pilT | 920173..921210 (-) | 1038 | WP_000355489.1 | type IV pilus twitching motility protein PilT | Machinery gene |
| ACK8DA_RS04370 (ACK8DA_04370) | - | 921337..922029 (+) | 693 | WP_004748094.1 | YggS family pyridoxal phosphate-dependent enzyme | - |
| ACK8DA_RS04375 (ACK8DA_04375) | - | 922231..925827 (-) | 3597 | WP_004748092.1 | SbcC/MukB-like Walker B domain-containing protein | - |
| ACK8DA_RS04380 (ACK8DA_04380) | - | 925837..927105 (-) | 1269 | WP_004748090.1 | exonuclease SbcCD subunit D | - |
| ACK8DA_RS04385 (ACK8DA_04385) | - | 927270..927743 (+) | 474 | WP_000974355.1 | OsmC family protein | - |
| ACK8DA_RS04390 (ACK8DA_04390) | - | 927811..928188 (-) | 378 | WP_001059437.1 | VOC family protein | - |
| ACK8DA_RS04395 (ACK8DA_04395) | - | 928568..929020 (+) | 453 | WP_000164151.1 | ABZJ_00895 family protein | - |
| ACK8DA_RS04400 (ACK8DA_04400) | - | 929108..930013 (+) | 906 | WP_046693190.1 | TIGR01777 family oxidoreductase | - |
| ACK8DA_RS04405 (ACK8DA_04405) | - | 930005..931120 (-) | 1116 | WP_004748085.1 | NAD(P)/FAD-dependent oxidoreductase | - |
| ACK8DA_RS04410 (ACK8DA_04410) | hemB | 931146..932159 (-) | 1014 | WP_004748084.1 | porphobilinogen synthase | - |
| ACK8DA_RS04415 (ACK8DA_04415) | - | 932302..932799 (+) | 498 | WP_002156418.1 | thioesterase family protein | - |
| ACK8DA_RS04420 (ACK8DA_04420) | - | 933069..934220 (+) | 1152 | WP_004748081.1 | EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit | - |
| ACK8DA_RS04425 (ACK8DA_04425) | - | 934227..935750 (+) | 1524 | WP_004748079.1 | DHA2 family efflux MFS transporter permease subunit | - |
| ACK8DA_RS04430 (ACK8DA_04430) | ggt | 936054..938039 (+) | 1986 | WP_004748076.1 | gamma-glutamyltransferase | - |
| ACK8DA_RS04440 (ACK8DA_04440) | - | 938741..939970 (+) | 1230 | WP_031999186.1 | tyrosine-type recombinase/integrase | - |
| ACK8DA_RS04445 (ACK8DA_04445) | - | 940118..940885 (+) | 768 | WP_000578618.1 | hypothetical protein | - |
| ACK8DA_RS04450 (ACK8DA_04450) | - | 941095..941310 (+) | 216 | WP_000153154.1 | hypothetical protein | - |
| ACK8DA_RS04455 (ACK8DA_04455) | - | 941313..941522 (+) | 210 | WP_001016479.1 | helix-turn-helix domain-containing protein | - |
| ACK8DA_RS04460 (ACK8DA_04460) | - | 941519..942073 (+) | 555 | WP_001988245.1 | Bro-N domain-containing protein | - |
| ACK8DA_RS04465 (ACK8DA_04465) | - | 942066..942434 (+) | 369 | WP_001046481.1 | hypothetical protein | - |
| ACK8DA_RS04470 (ACK8DA_04470) | - | 942444..942749 (+) | 306 | WP_000787149.1 | hypothetical protein | - |
| ACK8DA_RS04475 (ACK8DA_04475) | - | 942871..943149 (+) | 279 | WP_001064707.1 | hypothetical protein | - |
| ACK8DA_RS04480 (ACK8DA_04480) | - | 943162..945627 (+) | 2466 | WP_001022397.1 | DUF927 domain-containing protein | - |
| ACK8DA_RS04485 (ACK8DA_04485) | - | 945961..946077 (+) | 117 | WP_001992235.1 | single-stranded DNA-binding protein | - |
| ACK8DA_RS04490 (ACK8DA_04490) | - | 946300..947742 (+) | 1443 | WP_000003535.1 | hypothetical protein | - |
| ACK8DA_RS04495 (ACK8DA_04495) | ssb | 947724..947825 (+) | 102 | WP_168713084.1 | single-stranded DNA-binding protein | Machinery gene |
Sequence
Protein
Download Length: 372 a.a. Molecular weight: 41896.10 Da Isoelectric Point: 6.6406
>NTDB_id=1093202 ACK8DA_RS04360 WP_000347040.1 919027..920145(-) (pilU) [Acinetobacter baumannii strain MRSN122172]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ
Nucleotide
Download Length: 1119 bp
>NTDB_id=1093202 ACK8DA_RS04360 WP_000347040.1 919027..920145(-) (pilU) [Acinetobacter baumannii strain MRSN122172]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAGGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTCATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAGGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTCATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilU | Acinetobacter baylyi ADP1 |
75.926 |
100 |
0.772 |
| pilU | Pseudomonas stutzeri DSM 10701 |
69.022 |
98.925 |
0.683 |
| pilU | Vibrio cholerae strain A1552 |
52.973 |
99.462 |
0.527 |
| pilT | Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 |
42.197 |
93.011 |
0.392 |