Detailed information NTG1092729

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMFG5_RS04430
• Genome accession: NZ_CP179707
• Coordinates: 929671..930789 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain PR2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 924671..935789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMFG5_RS04400 (ACMFG5_04400)	-	925500..926348 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
ACMFG5_RS04405 (ACMFG5_04405)	-	926601..927056 (+)	456	WP_000782976.1	bacteriohemerythrin	-
ACMFG5_RS04410 (ACMFG5_04410)	-	927209..928276 (+)	1068	WP_002019572.1	hypothetical protein	-
ACMFG5_RS04415 (ACMFG5_04415)	-	928276..928599 (+)	324	WP_000442587.1	RnfH family protein	-
ACMFG5_RS04420 (ACMFG5_04420)	-	928654..929052 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACMFG5_RS04425 (ACMFG5_04425)	fur	929164..929601 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACMFG5_RS04430 (ACMFG5_04430)	pilU	929671..930789 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMFG5_RS04435 (ACMFG5_04435)	pilT	930817..931854 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACMFG5_RS04440 (ACMFG5_04440)	-	931981..932673 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=1092729 ACMFG5_RS04430 WP_000347039.1 929671..930789(-) (pilU) [Acinetobacter baumannii strain PR2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1092729 ACMFG5_RS04430 WP_000347039.1 929671..930789(-) (pilU) [Acinetobacter baumannii strain PR2]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395