Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACLSSQ_RS15415 Genome accession   NZ_CP179682
Coordinates   3237676..3238530 (+) Length   284 a.a.
NCBI ID   WP_130459326.1    Uniprot ID   -
Organism   Azospira sp. APE16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3224203..3237673 3237676..3238530 flank 3


Gene organization within MGE regions


Location: 3224203..3238530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLSSQ_RS15345 (ACLSSQ_15345) - 3224203..3224703 (-) 501 WP_416244298.1 DUF6776 family protein -
  ACLSSQ_RS15350 (ACLSSQ_15350) rpsI 3225147..3225539 (-) 393 WP_014236432.1 30S ribosomal protein S9 -
  ACLSSQ_RS15355 (ACLSSQ_15355) rplM 3225549..3225977 (-) 429 WP_014236431.1 50S ribosomal protein L13 -
  ACLSSQ_RS15360 (ACLSSQ_15360) - 3226478..3226894 (+) 417 WP_130459322.1 OsmC family protein -
  ACLSSQ_RS15365 (ACLSSQ_15365) coq7 3226932..3227555 (-) 624 WP_152090511.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  ACLSSQ_RS15370 (ACLSSQ_15370) - 3227577..3228137 (-) 561 WP_232058806.1 CNP1-like family protein -
  ACLSSQ_RS15375 (ACLSSQ_15375) - 3228194..3228805 (-) 612 WP_014236427.1 CBS domain-containing protein -
  ACLSSQ_RS15380 (ACLSSQ_15380) - 3228860..3229384 (-) 525 WP_014236426.1 RNA pyrophosphohydrolase -
  ACLSSQ_RS15385 (ACLSSQ_15385) - 3229544..3231256 (+) 1713 WP_416243166.1 proline--tRNA ligase -
  ACLSSQ_RS15390 (ACLSSQ_15390) - 3231244..3231852 (+) 609 WP_130459324.1 lytic transglycosylase domain-containing protein -
  ACLSSQ_RS15395 (ACLSSQ_15395) ffh 3232170..3233525 (-) 1356 WP_130459325.1 signal recognition particle protein -
  ACLSSQ_RS15400 (ACLSSQ_15400) - 3233571..3234410 (+) 840 WP_416243167.1 cytochrome C assembly family protein -
  ACLSSQ_RS15405 (ACLSSQ_15405) pilB 3234686..3236404 (+) 1719 WP_014236421.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLSSQ_RS15410 (ACLSSQ_15410) pilC 3236429..3237673 (+) 1245 WP_014236420.1 type II secretion system F family protein Machinery gene
  ACLSSQ_RS15415 (ACLSSQ_15415) pilD 3237676..3238530 (+) 855 WP_130459326.1 prepilin peptidase Machinery gene

Sequence

Protein


Download         Length: 284 a.a.        Molecular weight: 30758.55 Da        Isoelectric Point: 7.6890

>NTDB_id=1092567 ACLSSQ_RS15415 WP_130459326.1 3237676..3238530(+) (pilD) [Azospira sp. APE16]
MEFLLLESPALASAVAGILGLLVGSFLNVVIHRLPRMMERDWHLQCAELRGETAPEKERLSLAAPASRCPHCGHAIRAWE
NIPVLSFLLLKGRCSGCQAPISLRYPLVEAFTGLLSAFTVWHFGPTLAAAAALLLLWAMVALTGIDFDTQLLPDSITLPL
VWLGLLFNISGTFTDISSAVIGAMVGYLSLWSVYWLFKLATGKEGMGYGDFKLLAAIGAWLGWQMLPLTILLSSLVGAVV
GVALIVLARRGRNVPIPFGPYLAAAGLLALYWGQELTQSYLRLL

Nucleotide


Download         Length: 855 bp        

>NTDB_id=1092567 ACLSSQ_RS15415 WP_130459326.1 3237676..3238530(+) (pilD) [Azospira sp. APE16]
ATGGAATTTTTACTACTGGAAAGTCCCGCCCTTGCCTCTGCTGTGGCCGGAATCCTGGGCCTCCTGGTCGGCAGCTTCCT
CAACGTCGTCATCCACCGCCTGCCACGGATGATGGAGCGGGACTGGCACCTGCAGTGCGCCGAACTGCGGGGGGAAACGG
CACCGGAAAAGGAACGGCTGAGCCTCGCGGCCCCGGCCTCCCGCTGCCCCCATTGCGGCCACGCCATCCGGGCCTGGGAG
AACATCCCGGTACTCAGCTTCCTGCTCCTCAAAGGCCGCTGTTCCGGTTGCCAGGCCCCGATTTCCCTGCGCTACCCGTT
GGTGGAGGCCTTCACGGGCCTGCTCTCCGCCTTCACGGTATGGCACTTCGGCCCGACCCTGGCAGCGGCTGCCGCCCTGT
TGCTGCTGTGGGCCATGGTGGCCCTGACCGGCATCGACTTCGACACCCAGCTGCTGCCGGACTCCATCACCCTGCCCCTG
GTCTGGCTCGGCCTGCTGTTCAACATCTCCGGCACCTTCACCGACATCAGTTCCGCCGTCATCGGCGCCATGGTCGGCTA
CCTGTCCCTGTGGAGCGTCTACTGGCTGTTCAAGCTGGCCACCGGCAAGGAAGGCATGGGCTACGGCGACTTCAAGCTGC
TGGCGGCCATCGGCGCCTGGCTCGGCTGGCAGATGCTGCCCCTGACCATCCTGCTCTCCTCCCTGGTCGGTGCCGTCGTC
GGCGTGGCCCTGATCGTGCTGGCCCGCCGGGGCCGCAACGTGCCCATTCCCTTCGGTCCCTATCTGGCTGCTGCAGGCCT
GCTGGCCCTGTACTGGGGCCAGGAACTGACCCAGTCCTACCTGCGGCTGCTCTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)

Type IV leader peptidase family (Peptidase_A24)

(133-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

54.015

96.479

0.521
  pilD Vibrio cholerae strain A1552

53.623

97.183

0.521
  pilD Acinetobacter nosocomialis M2

53.65

96.479

0.518
  pilD Vibrio campbellii strain DS40M4

52.899

97.183

0.514
  pilD Neisseria gonorrhoeae MS11

49.627

94.366

0.468

Multiple sequence alignment