Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACKW6E_RS13575 Genome accession   NZ_CP178915
Coordinates   2982522..2983580 (-) Length   352 a.a.
NCBI ID   WP_054668759.1    Uniprot ID   -
Organism   Stenotrophomonas acidaminiphila strain ZCMU-Sa01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2977522..2988580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6E_RS13550 (ACKW6E_13550) - 2977614..2978624 (-) 1011 WP_413975161.1 AAA family ATPase -
  ACKW6E_RS13555 (ACKW6E_13555) - 2978739..2980631 (-) 1893 WP_056931187.1 type IV pilus secretin PilQ -
  ACKW6E_RS13560 (ACKW6E_13560) - 2980653..2981189 (-) 537 WP_056931186.1 pilus assembly protein PilP -
  ACKW6E_RS13565 (ACKW6E_13565) - 2981189..2981866 (-) 678 WP_056931185.1 type 4a pilus biogenesis protein PilO -
  ACKW6E_RS13570 (ACKW6E_13570) - 2981863..2982522 (-) 660 WP_413975162.1 PilN domain-containing protein -
  ACKW6E_RS13575 (ACKW6E_13575) pilM 2982522..2983580 (-) 1059 WP_054668759.1 pilus assembly protein PilM Machinery gene
  ACKW6E_RS13580 (ACKW6E_13580) - 2983798..2986230 (+) 2433 WP_413975163.1 penicillin-binding protein 1A -
  ACKW6E_RS13585 (ACKW6E_13585) - 2986309..2986935 (+) 627 WP_413975164.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37395.95 Da        Isoelectric Point: 4.4316

>NTDB_id=1092446 ACKW6E_RS13575 WP_054668759.1 2982522..2983580(-) (pilM) [Stenotrophomonas acidaminiphila strain ZCMU-Sa01]
MGLIPKSQPPIIGVDISSTAVKLLQLARSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVARSGTRAKGAAAA
VAGSAVITKVIPMPADLDEADLEAQVELEAVNYIPYPIEEVSLDFEVLGPVPNNPEMVQVLLAASRSENVELRQSALELG
GLVAKVMDVEAFAIENAFALVASELPVASDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEVEVLEPFKEATVQQIGRLLQFFYAGSEFNRVDHIVLAGGCASLPGLPVMVEEQLGVSTSVANPLA
QMTLGQKVQAHALAQDAPALMIATGLALRGFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1092446 ACKW6E_RS13575 WP_054668759.1 2982522..2983580(-) (pilM) [Stenotrophomonas acidaminiphila strain ZCMU-Sa01]
GTGGGGCTTATCCCAAAGAGTCAACCACCGATCATCGGTGTTGACATCAGCTCGACTGCGGTAAAGCTGCTTCAGCTTGC
CCGCAGCGGCAATCGTTTTCGCGTGGAACATTACGCCGTGGAACCACTTCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TCGAGGTCGAGGCGGTCGGCGAGGCCATACGCCGGGCCGTGGCGCGCTCGGGAACCCGGGCCAAGGGGGCAGCTGCCGCG
GTCGCCGGTTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGACCTGGACGAGGCCGACCTGGAAGCCCAGGTCGA
GCTGGAGGCGGTGAACTACATCCCGTATCCGATCGAGGAAGTGAGCCTGGACTTCGAGGTGCTCGGGCCGGTGCCCAACA
ACCCGGAAATGGTCCAGGTGCTGTTGGCGGCCTCGCGCTCGGAGAACGTCGAACTGCGCCAGTCGGCGCTGGAGCTGGGC
GGGCTGGTGGCCAAGGTCATGGACGTGGAAGCGTTCGCGATCGAGAACGCCTTTGCGCTGGTGGCCAGCGAACTGCCGGT
GGCCAGTGACGGCGTGGTCGCGCTGGTGGACATCGGCGCCACCATGACCACGCTCAACGTCCTGCGTGGCGGGCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGCTCACCGACGAGGTGATGCGCCGCTACGGGCTGAGCTACGAGGAA
GCCGGGCTGGCCAAGCGCCAGGGCGGGCTGCCGGAGAGCTACGAGGTGGAAGTGCTGGAGCCGTTCAAGGAGGCCACGGT
CCAGCAGATCGGCCGCCTGCTGCAGTTCTTCTATGCCGGCAGCGAGTTCAATCGCGTCGATCACATCGTGCTGGCCGGTG
GCTGCGCTTCGCTGCCCGGGCTGCCGGTGATGGTCGAGGAACAACTGGGGGTGAGCACGTCGGTGGCCAATCCGCTGGCG
CAGATGACGCTGGGACAGAAGGTGCAGGCGCATGCGCTGGCCCAGGATGCCCCGGCGCTGATGATCGCCACCGGCCTGGC
CCTGCGGGGGTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50

100

0.5
  comM Acinetobacter nosocomialis M2

50

100

0.5
  comM Acinetobacter baylyi ADP1

50.292

97.159

0.489
  pilM Legionella pneumophila strain ERS1305867

46.571

99.432

0.463

Multiple sequence alignment