Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACL6EO_RS12180 Genome accession   NZ_CP178718
Coordinates   2513466..2514536 (-) Length   356 a.a.
NCBI ID   WP_413383808.1    Uniprot ID   -
Organism   Bacillus velezensis strain SY1154     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2508466..2519536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL6EO_RS12130 (ACL6EO_12130) sipW 2508525..2509109 (-) 585 WP_012117977.1 signal peptidase I SipW -
  ACL6EO_RS12135 (ACL6EO_12135) tapA 2509081..2509752 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  ACL6EO_RS12140 (ACL6EO_12140) - 2510011..2510340 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  ACL6EO_RS12145 (ACL6EO_12145) - 2510381..2510560 (-) 180 WP_003153093.1 YqzE family protein -
  ACL6EO_RS12150 (ACL6EO_12150) comGG 2510617..2510994 (-) 378 WP_276350926.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACL6EO_RS12155 (ACL6EO_12155) comGF 2510995..2511390 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  ACL6EO_RS12160 (ACL6EO_12160) comGE 2511404..2511718 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACL6EO_RS12165 (ACL6EO_12165) comGD 2511702..2512139 (-) 438 WP_095061019.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACL6EO_RS12170 (ACL6EO_12170) comGC 2512129..2512437 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  ACL6EO_RS12175 (ACL6EO_12175) comGB 2512442..2513479 (-) 1038 WP_053284924.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACL6EO_RS12180 (ACL6EO_12180) comGA 2513466..2514536 (-) 1071 WP_413383808.1 competence type IV pilus ATPase ComGA Machinery gene
  ACL6EO_RS12185 (ACL6EO_12185) - 2514729..2515679 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  ACL6EO_RS12190 (ACL6EO_12190) - 2515825..2517126 (+) 1302 WP_029973873.1 hemolysin family protein -
  ACL6EO_RS12195 (ACL6EO_12195) - 2517153..2517989 (-) 837 WP_276350928.1 STAS domain-containing protein -
  ACL6EO_RS12205 (ACL6EO_12205) - 2518196..2518576 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACL6EO_RS12210 (ACL6EO_12210) - 2518813..2519058 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40555.85 Da        Isoelectric Point: 9.3485

>NTDB_id=1090933 ACL6EO_RS12180 WP_413383808.1 2513466..2514536(-) (comGA) [Bacillus velezensis strain SY1154]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSPGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1090933 ACL6EO_RS12180 WP_413383808.1 2513466..2514536(-) (comGA) [Bacillus velezensis strain SY1154]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCCCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment