Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACL1XL_RS13110 Genome accession   NZ_CP178592
Coordinates   2634375..2636084 (-) Length   569 a.a.
NCBI ID   WP_215861867.1    Uniprot ID   -
Organism   Acidithiobacillus ferriphilus strain QBS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2629375..2641084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL1XL_RS13090 (ACL1XL_13090) coaE 2629896..2630498 (-) 603 WP_215861727.1 dephospho-CoA kinase -
  ACL1XL_RS13095 (ACL1XL_13095) - 2630499..2631299 (-) 801 WP_064220377.1 prepilin peptidase -
  ACL1XL_RS13100 (ACL1XL_13100) pilR 2631296..2632654 (-) 1359 WP_215861728.1 sigma-54-dependent transcriptional regulator Regulator
  ACL1XL_RS13105 (ACL1XL_13105) - 2632651..2634249 (-) 1599 WP_413323291.1 two-component system sensor histidine kinase NtrB -
  ACL1XL_RS13110 (ACL1XL_13110) pilB 2634375..2636084 (-) 1710 WP_215861867.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACL1XL_RS13115 (ACL1XL_13115) aroE 2636054..2636953 (-) 900 WP_409409675.1 shikimate dehydrogenase -
  ACL1XL_RS13120 (ACL1XL_13120) - 2636931..2638094 (-) 1164 WP_409409674.1 anhydro-N-acetylmuramic acid kinase -
  ACL1XL_RS13125 (ACL1XL_13125) - 2638162..2638650 (-) 489 WP_064218850.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  ACL1XL_RS13130 (ACL1XL_13130) hemH 2638702..2639724 (-) 1023 WP_233470670.1 ferrochelatase -
  ACL1XL_RS13135 (ACL1XL_13135) xth 2639721..2640494 (-) 774 WP_278666306.1 exodeoxyribonuclease III -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62896.65 Da        Isoelectric Point: 6.4404

>NTDB_id=1090264 ACL1XL_RS13110 WP_215861867.1 2634375..2636084(-) (pilB) [Acidithiobacillus ferriphilus strain QBS1]
MATNPAHITLTPVLRALVSNGLSDEAQLQRLAADPARGKMPLLFYAVERGAVPASVLMAHLSARYNMPMLDLDAVMINEL
LIRKLDKGLMTRYLVLPLSKHGDTLYLAMADPTDFKAVEDVKFNTGLQVLPILVEANKLVKAVNAAANSMQGGIDDVFMD
KPRGEEEQEEFDLAQESDGSVEDAPVVRFARQLLLDAIQRGVSDIHLEPYEKDFRVRYRLDGVLQDVLHPPVALRDGVTS
RLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETIVLRLLDPASSKVPIEQLGFLPDQRKAFEEAIHRP
YGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLNFATALRSFLRQDPDIIMVGEVR
DLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGGVHLVMAQRLARKLCPHCKKPLRIPEQALIEAGF
THEDLQGWRPLGAVGCDQCNNTGYKGRMGLYQVMPVSDAMREIIMRGGTSIDLARQATQEGVLTMRQNGLRRVREGVTSL
EEVLRVTNL

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1090264 ACL1XL_RS13110 WP_215861867.1 2634375..2636084(-) (pilB) [Acidithiobacillus ferriphilus strain QBS1]
ATGGCTACAAATCCAGCACACATCACGTTGACGCCCGTATTGCGGGCGTTGGTCAGCAACGGGTTAAGCGATGAGGCGCA
GTTGCAACGCCTTGCCGCCGATCCGGCGCGTGGCAAGATGCCGCTGCTGTTTTATGCGGTTGAAAGGGGTGCCGTTCCTG
CCTCTGTGCTTATGGCGCACCTTTCTGCGCGCTATAACATGCCCATGCTTGATCTGGATGCGGTCATGATTAATGAGTTG
CTTATTCGGAAGCTCGACAAAGGACTGATGACCCGCTATCTGGTGCTGCCCTTGTCGAAGCATGGGGACACCCTCTACCT
CGCCATGGCGGACCCTACGGATTTTAAGGCGGTGGAGGATGTGAAGTTCAACACGGGTCTGCAGGTGTTGCCAATACTGG
TCGAGGCCAATAAGCTGGTAAAGGCGGTGAATGCCGCAGCCAACAGTATGCAAGGCGGGATTGACGATGTTTTCATGGAT
AAACCGCGCGGCGAAGAAGAGCAGGAAGAATTCGATCTTGCCCAGGAGAGTGATGGCAGTGTCGAGGATGCACCCGTGGT
GCGTTTTGCCCGGCAATTGCTGTTGGATGCCATTCAACGCGGGGTTTCCGACATTCATCTGGAACCGTACGAAAAGGACT
TTCGGGTGCGTTACCGACTCGATGGCGTATTGCAGGATGTGTTGCATCCGCCGGTGGCGCTGCGTGACGGTGTCACTTCG
CGCCTGAAGATTCTCTGTCGTCTGGATATTTCTGAGCGGCGTTTACCCCAGGATGGCCGTTTGCGGGTCAGGGTGCCGCC
GGCGCGGGTTATTGATTTCCGGGTTTCTTTTTTGCCCACCAGTTTTGGCGAAACCATTGTGCTGCGCTTGCTGGATCCGG
CCAGTTCCAAGGTGCCCATAGAACAACTGGGCTTTTTGCCGGACCAGCGCAAAGCTTTTGAAGAGGCCATCCACCGGCCC
TACGGTATGATCCTGGTCACCGGCCCCACGGGTTCGGGTAAAACGACGACTCTTTACACCGCGCTGAATATTCTCAATAC
CGGCGACTGCAATATCAGTACCGCAGAGGATCCTGTGGAAATCCCGGTGTACGGGATTAATCAGGTGAATATCAACGAGC
GTATCGGTCTCAACTTTGCCACCGCCTTGCGTTCCTTTCTGCGTCAGGACCCTGACATCATTATGGTCGGTGAGGTGCGT
GATCTGGAAACCGCGGAAACGGCCATCAAAGCCGCCCAGACGGGTCATCTGGTGTTGGCCACCCTGCATACCAATGATGC
ACCACAGAGTCTCACCCGTCTGGAAAATATGGGCATCCCTACCTATAACATAGCAGGCGGTGTGCACTTGGTGATGGCGC
AGCGACTCGCACGCAAACTCTGCCCGCATTGTAAAAAGCCGCTGCGCATTCCCGAACAGGCCCTGATTGAGGCGGGATTT
ACGCACGAGGATCTGCAGGGTTGGCGTCCTCTGGGCGCGGTGGGCTGTGATCAGTGCAACAACACCGGATATAAAGGCCG
GATGGGACTCTATCAGGTGATGCCGGTGAGCGATGCTATGCGTGAAATCATTATGCGCGGTGGTACCTCCATCGATCTGG
CGCGGCAGGCGACACAAGAGGGAGTGCTGACCATGCGGCAAAACGGATTGAGGCGGGTCAGGGAGGGTGTCACTAGTCTT
GAAGAGGTCCTGCGGGTGACCAATCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

48.354

100

0.49
  pilB Acinetobacter baumannii D1279779

48.063

99.824

0.48
  pilB Acinetobacter baylyi ADP1

46.479

99.824

0.464
  pilF Neisseria gonorrhoeae MS11

45.276

98.594

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

43.106

100

0.434
  pilB Vibrio cholerae strain A1552

46.641

91.564

0.427
  pilB Vibrio campbellii strain DS40M4

44.828

91.74

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

93.322

0.371

Multiple sequence alignment