Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACLWNE_RS02260 Genome accession   NZ_CP178485
Coordinates   412711..414165 (-) Length   484 a.a.
NCBI ID   WP_413217666.1    Uniprot ID   -
Organism   Thermus oshimai strain Blanco     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407711..419165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLWNE_RS02230 (ACLWNE_02230) - 407822..408181 (+) 360 WP_016328699.1 DUF4870 domain-containing protein -
  ACLWNE_RS02235 (ACLWNE_02235) - 408162..408533 (-) 372 WP_018460595.1 4'-phosphopantetheinyl transferase superfamily protein -
  ACLWNE_RS02240 (ACLWNE_02240) rsmF 408568..409932 (+) 1365 WP_018460596.1 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF -
  ACLWNE_RS02245 (ACLWNE_02245) - 409927..410952 (-) 1026 WP_413217380.1 3-dehydroquinate synthase family protein -
  ACLWNE_RS02250 (ACLWNE_02250) - 410930..411484 (-) 555 WP_413217381.1 shikimate kinase -
  ACLWNE_RS02255 (ACLWNE_02255) aroC 411484..412635 (-) 1152 WP_413217382.1 chorismate synthase -
  ACLWNE_RS02260 (ACLWNE_02260) pilQ 412711..414165 (-) 1455 WP_413217666.1 secretin N-terminal domain-containing protein Machinery gene
  ACLWNE_RS02265 (ACLWNE_02265) - 414146..414220 (+) 75 WP_413217667.1 hypothetical protein -
  ACLWNE_RS02270 (ACLWNE_02270) pilW 414978..415922 (-) 945 WP_413217383.1 competence protein Machinery gene
  ACLWNE_RS02275 (ACLWNE_02275) pilO 415919..416482 (-) 564 WP_187288203.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLWNE_RS02280 (ACLWNE_02280) pilN 416523..417149 (-) 627 WP_016328708.1 hypothetical protein Machinery gene
  ACLWNE_RS02285 (ACLWNE_02285) pilM 417142..418287 (-) 1146 WP_018460601.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 52078.78 Da        Isoelectric Point: 5.1418

>NTDB_id=1089690 ACLWNE_RS02260 WP_413217666.1 412711..414165(-) (pilQ) [Thermus oshimai strain Blanco]
MPGAQITPLPTDPKRAVVLATEADHAKLAGLLQAADLPKAVRRVYTLQNTTLEAVGERLRALLSKELPAARLEAIPGNPK
ALLLEAPESDQALFAELLKAVDVAEAPPPPPQEATVRRLFPLRFADAEKVAPFLAREVPGVVVQTVPGQRILSVRGTEAA
LKEVEALLLQIDRAPEQGPALFQRTYQLSNAKAKDLAAVLQEALKAKTGPQPQAQGQEATATPTATVVADERTNTLIVTG
TAEDLALVEGLIPRLDQAVPQVNLRVRIQEVQSNFSRALGLKWNTIAGGNVAASIVDAGLSLIFDSTRSLAALNIMATLD
ALQRQGLSRALRDVNQTVLNNQTARLQSGETFLIRRVVGDRVERVPFDIGIIVEVTPQITADGQILLNIKAEVSGNVQRN
PVDGDVDRFTKQVVTTTLRVRDGQTVVLGGLTSQENNETVQGVPFLMDIPLIGELFKQRTGETTDRELLVVITADVLKET
ASRP

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=1089690 ACLWNE_RS02260 WP_413217666.1 412711..414165(-) (pilQ) [Thermus oshimai strain Blanco]
GTGCCGGGGGCCCAGATCACCCCCCTCCCCACCGACCCCAAGCGGGCCGTGGTCCTGGCCACGGAAGCCGACCACGCCAA
GCTCGCCGGGCTCCTCCAGGCCGCCGACCTGCCCAAGGCGGTCCGCCGGGTCTACACCCTGCAGAACACCACCCTCGAGG
CCGTAGGGGAAAGGCTAAGGGCCCTCCTCTCCAAGGAGCTTCCAGCCGCCCGCCTGGAGGCCATCCCCGGCAACCCCAAA
GCCCTCCTCCTGGAAGCCCCCGAAAGCGACCAGGCCCTCTTCGCCGAACTCCTCAAAGCCGTGGACGTGGCGGAGGCCCC
GCCCCCCCCTCCCCAGGAGGCCACGGTGCGCAGGCTCTTCCCCCTGCGCTTCGCCGACGCGGAGAAGGTGGCCCCCTTCC
TGGCCCGGGAGGTGCCGGGCGTGGTGGTCCAGACCGTGCCCGGGCAGCGGATCCTCTCCGTGCGGGGCACGGAAGCGGCC
CTAAAGGAGGTGGAGGCCCTCCTTTTGCAGATTGACCGGGCCCCGGAGCAAGGCCCCGCCCTCTTCCAGCGCACCTACCA
GCTCTCCAACGCGAAGGCCAAGGACCTGGCCGCCGTTCTGCAGGAGGCCCTGAAGGCCAAGACCGGCCCCCAGCCCCAGG
CCCAAGGCCAGGAGGCCACGGCCACCCCCACCGCCACCGTGGTGGCCGACGAGCGCACCAACACCCTCATCGTCACGGGG
ACCGCGGAGGACCTGGCCCTGGTGGAAGGCCTCATCCCCCGGCTGGACCAGGCGGTGCCCCAGGTGAACCTGAGGGTGCG
CATCCAGGAGGTGCAGAGCAACTTCAGCCGCGCCCTGGGCCTCAAGTGGAACACCATCGCCGGGGGGAACGTGGCCGCCA
GCATCGTGGACGCCGGGCTTTCCCTCATCTTTGACTCCACCAGGAGCCTCGCCGCCCTCAACATCATGGCCACCCTGGAC
GCCCTGCAGCGGCAGGGGCTTTCCCGGGCCCTAAGGGACGTGAACCAGACGGTGCTCAACAACCAGACCGCCCGCCTCCA
GTCCGGGGAGACCTTCCTCATCCGCCGGGTGGTGGGGGACCGGGTGGAGCGAGTGCCCTTTGACATCGGCATCATCGTGG
AGGTCACGCCCCAGATCACCGCGGACGGCCAGATCCTCCTCAACATCAAGGCGGAGGTCTCGGGGAACGTGCAGCGGAAC
CCCGTGGACGGGGACGTGGACCGCTTCACCAAGCAGGTGGTGACCACCACCCTCAGGGTCCGGGACGGCCAGACCGTGGT
CCTGGGGGGCCTCACCTCCCAGGAGAACAACGAAACCGTCCAGGGGGTGCCCTTCCTGATGGACATCCCCCTCATCGGGG
AGCTCTTCAAGCAACGGACGGGGGAAACCACCGACCGGGAACTCCTGGTGGTCATCACCGCGGACGTGCTGAAGGAAACG
GCGAGCAGGCCCTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(321-478)

Bacterial type II/III secretion system short domain (Secretin_N)

(118-177)

Bacterial type II/III secretion system short domain (Secretin_N)

(184-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Thermus thermophilus HB27

78.926

100

0.789

Multiple sequence alignment