Detailed information NTG1089694

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACLWNE_RS02285
• Genome accession: NZ_CP178485
• Coordinates: 417142..418287 (-)
• Length: 381 a.a.
• NCBI ID: WP_018460601.1
• Uniprot ID: -
• Organism: Thermus oshimai strain Blanco
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 412142..423287

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLWNE_RS02260 (ACLWNE_02260)	pilQ	412711..414165 (-)	1455	WP_413217666.1	secretin N-terminal domain-containing protein	Machinery gene
ACLWNE_RS02265 (ACLWNE_02265)	-	414146..414220 (+)	75	WP_413217667.1	hypothetical protein	-
ACLWNE_RS02270 (ACLWNE_02270)	pilW	414978..415922 (-)	945	WP_413217383.1	competence protein	Machinery gene
ACLWNE_RS02275 (ACLWNE_02275)	pilO	415919..416482 (-)	564	WP_187288203.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACLWNE_RS02280 (ACLWNE_02280)	pilN	416523..417149 (-)	627	WP_016328708.1	hypothetical protein	Machinery gene
ACLWNE_RS02285 (ACLWNE_02285)	pilM	417142..418287 (-)	1146	WP_018460601.1	type IV pilus assembly protein PilM	Machinery gene
ACLWNE_RS02290 (ACLWNE_02290)	-	418472..419476 (-)	1005	WP_339956838.1	homoisocitrate dehydrogenase	-
ACLWNE_RS02295 (ACLWNE_02295)	-	419522..420028 (-)	507	WP_413217668.1	DUF5317 domain-containing protein	-
ACLWNE_RS02300 (ACLWNE_02300)	-	420064..421458 (-)	1395	WP_413217384.1	HD-GYP domain-containing protein	-
ACLWNE_RS02305 (ACLWNE_02305)	-	421495..422586 (-)	1092	WP_014514485.1	IS4 family transposase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 41756.32 Da
• Isoelectric Point: 4.4801

>NTDB_id=1089694 ACLWNE_RS02285 WP_018460601.1 417142..418287(-) (pilM) [Thermus oshimai strain Blanco]
MVQRLFRQGALSRLFKPRVEALGLEIGAAHLKLVELSGNPPALKAWAMRPTPPGTLVDGLIAEPSALAQELRELLAEART
KKRYVVTAAPNLSVILRTVQVPKMPAKEMEEAVRWEAERYIPFPIDEVVLDYAPLTPLEEAEEGEQVEVMVGAARKESVA
GLIEALRGAGLIPVVVDVKPFAGLYPLEEKLTEGPVTLSVDIGAESTSLVLTQGDRPLYVRILTLSGKDFTEAIGKAFGL
DLLSAEEVKRTYGLATIPTEDEELLLDFDAERERYSPARIYDAIRPVLVELTQEIRRSLEFFRVQMGDLQPEVGYLTGGG
SKLRGLPTLLTDTLGVSFEVPNPWEGIAVDPKRFDLEELKGLAPEFMVPVGLALRGVSPLD

Nucleotide

• Length: 1146 bp

>NTDB_id=1089694 ACLWNE_RS02285 WP_018460601.1 417142..418287(-) (pilM) [Thermus oshimai strain Blanco]
GTGGTTCAAAGGCTCTTTCGTCAGGGCGCACTGAGCCGTCTATTCAAACCCCGGGTTGAGGCCCTGGGCCTGGAGATCGG
GGCGGCGCACCTCAAGCTGGTGGAGCTTTCCGGCAACCCCCCGGCCCTCAAGGCTTGGGCCATGCGGCCCACCCCCCCGG
GCACCCTCGTGGACGGGCTCATCGCCGAGCCCAGCGCCTTAGCTCAAGAACTCCGGGAGCTCCTCGCTGAGGCCAGGACC
AAGAAGCGCTACGTGGTCACCGCGGCCCCCAACCTCTCGGTCATCCTGCGCACGGTCCAGGTGCCCAAGATGCCCGCCAA
GGAGATGGAGGAGGCGGTGCGCTGGGAGGCGGAGCGCTACATCCCCTTCCCCATAGACGAGGTGGTCCTGGACTACGCCC
CCCTCACCCCCCTGGAGGAGGCCGAGGAGGGGGAGCAGGTGGAGGTCATGGTGGGGGCCGCCCGGAAGGAGAGCGTGGCG
GGGCTCATCGAGGCCCTGAGGGGGGCAGGGCTCATCCCGGTGGTGGTGGACGTGAAGCCCTTCGCGGGGCTCTACCCCTT
GGAGGAGAAGCTCACGGAGGGTCCGGTGACCCTTTCCGTGGACATCGGAGCGGAGAGCACCAGCCTGGTCCTCACCCAGG
GGGACCGGCCCCTTTACGTGCGCATCCTCACCCTCTCCGGCAAGGACTTCACCGAGGCCATCGGCAAGGCCTTCGGCCTG
GACCTCCTCAGCGCGGAGGAGGTCAAGCGCACCTATGGCCTGGCCACCATCCCCACGGAGGACGAGGAACTCCTCCTGGA
CTTTGACGCGGAGAGGGAACGGTACAGCCCGGCCCGCATCTATGACGCCATCCGCCCCGTGCTGGTGGAGCTCACCCAGG
AGATCCGGCGGAGCCTGGAGTTCTTCCGGGTGCAGATGGGGGACCTCCAGCCCGAGGTGGGCTACCTCACGGGGGGCGGG
AGCAAGCTCCGGGGCCTCCCCACCCTCCTCACGGACACCCTGGGGGTGAGCTTTGAGGTGCCCAACCCCTGGGAGGGCAT
CGCCGTGGACCCCAAGCGCTTTGACCTGGAAGAGCTCAAGGGCCTGGCCCCGGAGTTTATGGTGCCCGTGGGCCTGGCCT
TAAGGGGGGTGAGTCCGCTTGATTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (23-376)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	77.273	98.163	0.759