Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACLWNE_RS02275 Genome accession   NZ_CP178485
Coordinates   415919..416482 (-) Length   187 a.a.
NCBI ID   WP_187288203.1    Uniprot ID   -
Organism   Thermus oshimai strain Blanco     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 410919..421482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLWNE_RS02250 (ACLWNE_02250) - 410930..411484 (-) 555 WP_413217381.1 shikimate kinase -
  ACLWNE_RS02255 (ACLWNE_02255) aroC 411484..412635 (-) 1152 WP_413217382.1 chorismate synthase -
  ACLWNE_RS02260 (ACLWNE_02260) pilQ 412711..414165 (-) 1455 WP_413217666.1 secretin N-terminal domain-containing protein Machinery gene
  ACLWNE_RS02265 (ACLWNE_02265) - 414146..414220 (+) 75 WP_413217667.1 hypothetical protein -
  ACLWNE_RS02270 (ACLWNE_02270) pilW 414978..415922 (-) 945 WP_413217383.1 competence protein Machinery gene
  ACLWNE_RS02275 (ACLWNE_02275) pilO 415919..416482 (-) 564 WP_187288203.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLWNE_RS02280 (ACLWNE_02280) pilN 416523..417149 (-) 627 WP_016328708.1 hypothetical protein Machinery gene
  ACLWNE_RS02285 (ACLWNE_02285) pilM 417142..418287 (-) 1146 WP_018460601.1 type IV pilus assembly protein PilM Machinery gene
  ACLWNE_RS02290 (ACLWNE_02290) - 418472..419476 (-) 1005 WP_339956838.1 homoisocitrate dehydrogenase -
  ACLWNE_RS02295 (ACLWNE_02295) - 419522..420028 (-) 507 WP_413217668.1 DUF5317 domain-containing protein -
  ACLWNE_RS02300 (ACLWNE_02300) - 420064..421458 (-) 1395 WP_413217384.1 HD-GYP domain-containing protein -

Sequence

Protein


Download         Length: 187 a.a.        Molecular weight: 20245.24 Da        Isoelectric Point: 5.0686

>NTDB_id=1089692 ACLWNE_RS02275 WP_187288203.1 415919..416482(-) (pilO) [Thermus oshimai strain Blanco]
MALTVILALLWYFLLIAPIRQETEAVRNEIATLIPERDRGRQAQRALPELRAAIAELQAERQAFLRALPREERLGEVLNA
LLSEAARSGVTVRALNRSPTSAPVPEVRAVNLALSLEGPFPETYAYLRRLEGLSRFSSISGLSLAVAGEPSLNPTLNTSL
TLTLYMLAKDVEELEQASGTPGGGGGQ

Nucleotide


Download         Length: 564 bp        

>NTDB_id=1089692 ACLWNE_RS02275 WP_187288203.1 415919..416482(-) (pilO) [Thermus oshimai strain Blanco]
ATGGCCCTCACCGTGATCCTGGCCCTCCTCTGGTACTTCCTCCTCATCGCCCCCATCCGCCAGGAAACGGAGGCGGTGCG
GAACGAGATCGCCACCCTCATCCCCGAGCGGGACCGGGGGCGCCAGGCCCAAAGGGCCCTGCCAGAACTCCGGGCGGCCA
TTGCCGAGCTTCAGGCGGAGCGCCAAGCCTTCCTAAGGGCCCTTCCCCGGGAGGAGCGGCTTGGGGAGGTGCTGAACGCC
CTCCTCTCGGAGGCGGCCCGGAGCGGGGTGACCGTGCGGGCCCTAAACCGCTCCCCCACCTCCGCCCCTGTCCCGGAGGT
GCGGGCGGTGAACCTGGCCCTATCCCTGGAGGGCCCCTTCCCGGAAACCTACGCCTACCTCAGGCGCCTGGAGGGGCTTT
CCCGCTTCAGTTCCATCTCTGGGCTTTCCCTGGCGGTGGCCGGGGAACCTAGCCTGAACCCCACCCTGAACACCAGCCTG
ACCCTAACCCTCTACATGTTGGCCAAGGACGTGGAAGAACTGGAGCAGGCGTCCGGAACCCCGGGCGGGGGAGGTGGACA
GTGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(31-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Thermus thermophilus HB27

67.403

96.791

0.652

Multiple sequence alignment