Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACLSIV_RS02330 Genome accession   NZ_CP178385
Coordinates   463771..465045 (-) Length   424 a.a.
NCBI ID   WP_172506944.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain GCULR     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458771..470045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLSIV_RS02295 (ACLSIV_02295) obgE 459174..460493 (+) 1320 WP_096039988.1 GTPase ObgE -
  ACLSIV_RS02300 (ACLSIV_02300) - 460580..460756 (+) 177 WP_003137484.1 hypothetical protein -
  ACLSIV_RS02305 (ACLSIV_02305) - 460753..461739 (+) 987 WP_061773784.1 PhoH family protein -
  ACLSIV_RS02310 (ACLSIV_02310) - 461732..462190 (+) 459 WP_138492264.1 HIT family protein -
  ACLSIV_RS02315 (ACLSIV_02315) ybeY 462248..462739 (+) 492 WP_061773786.1 rRNA maturation RNase YbeY -
  ACLSIV_RS02320 (ACLSIV_02320) - 462723..463124 (+) 402 WP_138492265.1 diacylglycerol kinase family protein -
  ACLSIV_RS02325 (ACLSIV_02325) comFC 463127..463456 (-) 330 WP_163604800.1 ComF family protein Machinery gene
  ACLSIV_RS02330 (ACLSIV_02330) comFA/cflA 463771..465045 (-) 1275 WP_172506944.1 DEAD/DEAH box helicase Machinery gene
  ACLSIV_RS02335 (ACLSIV_02335) - 465089..465712 (+) 624 WP_096039992.1 YigZ family protein -
  ACLSIV_RS02345 (ACLSIV_02345) - 466537..467400 (+) 864 WP_167839777.1 S1 RNA-binding domain-containing protein -
  ACLSIV_RS02350 (ACLSIV_02350) msrA 467397..467918 (+) 522 WP_061773792.1 peptide-methionine (S)-S-oxide reductase MsrA -
  ACLSIV_RS02355 (ACLSIV_02355) - 467915..468124 (+) 210 WP_096039994.1 YozE family protein -
  ACLSIV_RS02360 (ACLSIV_02360) rplK 468366..468791 (+) 426 WP_003139488.1 50S ribosomal protein L11 -
  ACLSIV_RS02365 (ACLSIV_02365) rplA 468964..469650 (+) 687 WP_061773794.1 50S ribosomal protein L1 -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 47657.73 Da        Isoelectric Point: 8.8280

>NTDB_id=1089287 ACLSIV_RS02330 WP_172506944.1 463771..465045(-) (comFA/cflA) [Lactococcus raffinolactis strain GCULR]
MDNLYGRLLTRQELGDDFENLPKDTETFPGMSITPKSVTCHRCGTTSSLQAVKLEIPAYFCPECLHLGRVRSDELLYHLP
QQDFPPQDSLLWSGQLTPYQAEISKELMAAVDQKTQILVHAVTGAGKTEMIYAAVARVISSCGAVGIATPRTDVARELHA
RLSRDFSIPISLLHAESEPYFPTPLVISTTHQLLRFRHAFDLLIIDEVDAFPFADNDALYFAAEQSRKPTATLIYLTATS
TDKLDKLVKTGQLKQVSLSRRFHGNPLVVPKVIFSGSEKRIYRHIKKQRETGFPLLIFAPVIAFGQSFTERLRRLFPDEK
IGFVASTSEERAEEIDRFRQGQLTILVSTTILERGVTFPKVDVFVVNSHHRLFTKSSLIQIAGRAGRSPDRSTGLVYFFH
TGLTRDMTRAISEIRQMNRLGGFT

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=1089287 ACLSIV_RS02330 WP_172506944.1 463771..465045(-) (comFA/cflA) [Lactococcus raffinolactis strain GCULR]
ATGGATAACTTATACGGTCGCCTATTAACGCGACAAGAACTGGGGGATGACTTCGAAAATCTCCCAAAAGACACCGAGAC
CTTCCCTGGGATGTCAATCACACCAAAATCGGTGACTTGTCATCGATGCGGGACAACATCTTCTCTTCAAGCAGTTAAGT
TAGAGATTCCTGCTTACTTTTGTCCAGAGTGCCTTCACCTCGGACGGGTTCGGTCTGATGAATTACTCTACCACTTACCT
CAGCAAGATTTCCCACCTCAAGATTCCCTCCTTTGGAGCGGCCAACTCACGCCCTATCAAGCTGAAATTTCAAAGGAACT
GATGGCTGCAGTTGATCAGAAAACTCAGATTTTAGTCCATGCTGTTACAGGGGCTGGTAAAACTGAGATGATTTATGCTG
CCGTGGCTAGAGTCATCTCAAGTTGTGGGGCCGTTGGGATTGCGACGCCCAGGACCGACGTTGCCCGCGAACTACATGCC
AGATTATCTCGCGACTTTTCTATCCCAATCTCTCTCCTACACGCTGAATCAGAGCCTTACTTTCCGACACCACTCGTCAT
TTCAACCACCCACCAACTACTGCGCTTTCGCCATGCCTTTGATCTATTGATTATCGACGAAGTGGACGCCTTTCCCTTCG
CTGATAATGATGCCCTTTACTTTGCGGCTGAGCAGTCCCGAAAACCTACCGCAACGTTGATTTATCTAACCGCGACATCT
ACTGACAAACTCGATAAGCTGGTCAAAACTGGCCAGTTAAAACAGGTATCTTTGTCCCGTCGCTTTCATGGCAACCCACT
CGTTGTCCCAAAAGTCATCTTCAGTGGCAGCGAAAAACGCATCTACCGTCACATCAAAAAACAGCGGGAGACAGGTTTTC
CACTCCTCATTTTCGCGCCCGTCATTGCCTTTGGTCAATCGTTCACAGAGCGCTTGCGACGTCTGTTTCCTGACGAAAAA
ATTGGCTTTGTAGCCTCTACTAGTGAGGAGCGTGCTGAGGAGATTGACCGTTTTCGACAAGGGCAACTCACGATTTTAGT
TTCGACGACCATTTTAGAGCGAGGGGTGACTTTTCCTAAGGTTGATGTTTTTGTGGTCAATAGTCACCACCGTCTCTTTA
CCAAATCAAGTTTGATTCAGATTGCGGGAAGGGCTGGCCGAAGCCCTGATCGGTCGACTGGTCTGGTATACTTTTTTCAT
ACTGGCCTAACCAGAGACATGACCCGAGCTATTTCAGAAATTCGCCAGATGAATCGACTGGGAGGTTTTACATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(281-387)

Type III restriction enzyme, res subunit (ResIII)

(95-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

55.452

100

0.564
  comFA/cflA Streptococcus pneumoniae Rx1

55.374

100

0.559
  comFA/cflA Streptococcus pneumoniae D39

55.374

100

0.559
  comFA/cflA Streptococcus pneumoniae R6

55.374

100

0.559
  comFA/cflA Streptococcus pneumoniae TIGR4

55.14

100

0.557
  comFA/cflA Streptococcus mitis NCTC 12261

54.756

100

0.557
  comFA Lactococcus lactis subsp. cremoris KW2

56.633

92.453

0.524
  comFA Latilactobacillus sakei subsp. sakei 23K

36.322

100

0.373
  comFA Bacillus subtilis subsp. subtilis str. 168

36.779

98.113

0.361

Multiple sequence alignment