Detailed information NTG1089161

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: ACLNGX_RS12000
• Genome accession: NZ_CP178374
• Coordinates: 2538518..2539588 (-)
• Length: 356 a.a.
• NCBI ID: WP_003153083.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 2023
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2533518..2544588

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLNGX_RS11950 (ACLNGX_11950)	sipW	2533578..2534162 (-)	585	WP_060562614.1	signal peptidase I SipW	-
ACLNGX_RS11955 (ACLNGX_11955)	tapA	2534134..2534805 (-)	672	WP_071391613.1	amyloid fiber anchoring/assembly protein TapA	-
ACLNGX_RS11960 (ACLNGX_11960)	-	2535064..2535393 (+)	330	WP_071391612.1	DUF3889 domain-containing protein	-
ACLNGX_RS11965 (ACLNGX_11965)	-	2535433..2535612 (-)	180	WP_003153093.1	YqzE family protein	-
ACLNGX_RS11970 (ACLNGX_11970)	comGG	2535669..2536046 (-)	378	WP_071391611.1	competence type IV pilus minor pilin ComGG	Machinery gene
ACLNGX_RS11975 (ACLNGX_11975)	comGF	2536047..2536442 (-)	396	WP_071391610.1	competence type IV pilus minor pilin ComGF	-
ACLNGX_RS11980 (ACLNGX_11980)	comGE	2536456..2536770 (-)	315	WP_015388003.1	competence type IV pilus minor pilin ComGE	Machinery gene
ACLNGX_RS11985 (ACLNGX_11985)	comGD	2536754..2537191 (-)	438	WP_025852922.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACLNGX_RS11990 (ACLNGX_11990)	comGC	2537181..2537489 (-)	309	WP_003153087.1	competence type IV pilus major pilin ComGC	Machinery gene
ACLNGX_RS11995 (ACLNGX_11995)	comGB	2537494..2538531 (-)	1038	WP_063174751.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACLNGX_RS12000 (ACLNGX_12000)	comGA	2538518..2539588 (-)	1071	WP_003153083.1	competence type IV pilus ATPase ComGA	Machinery gene
ACLNGX_RS12005 (ACLNGX_12005)	-	2539780..2540730 (-)	951	WP_071391609.1	magnesium transporter CorA family protein	-
ACLNGX_RS12010 (ACLNGX_12010)	-	2540877..2542178 (+)	1302	WP_166706141.1	hemolysin family protein	-
ACLNGX_RS12015 (ACLNGX_12015)	-	2542216..2543046 (-)	831	WP_071391608.1	STAS domain-containing protein	-
ACLNGX_RS12025 (ACLNGX_12025)	-	2543251..2543631 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
ACLNGX_RS12030 (ACLNGX_12030)	-	2543868..2544113 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 40529.85 Da
• Isoelectric Point: 9.4729

>NTDB_id=1089161 ACLNGX_RS12000 WP_003153083.1 2538518..2539588(-) (comGA) [Bacillus velezensis strain 2023]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

• Length: 1071 bp

>NTDB_id=1089161 ACLNGX_RS12000 WP_003153083.1 2538518..2539588(-) (comGA) [Bacillus velezensis strain 2023]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGGCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGATCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GTGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGTGCCATATACAGACTGTTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTTACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (10-275)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.022	100	0.82
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374