Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilY1   Type   Machinery gene
Locus tag   ACKWDP_RS17730 Genome accession   NZ_CP178354
Coordinates   3639883..3643737 (-) Length   1284 a.a.
NCBI ID   WP_000768943.1    Uniprot ID   A0A0J0ZLJ4
Organism   Acinetobacter baumannii strain A297     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3634883..3648737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWDP_RS17695 (ACKWDP_17695) - 3635647..3636588 (+) 942 WP_085916958.1 lipase family alpha/beta hydrolase -
  ACKWDP_RS17700 (ACKWDP_17700) rplS 3636640..3637008 (-) 369 WP_000014562.1 50S ribosomal protein L19 -
  ACKWDP_RS17705 (ACKWDP_17705) trmD 3637216..3637956 (-) 741 WP_000464595.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ACKWDP_RS17710 (ACKWDP_17710) rimM 3638002..3638550 (-) 549 WP_000189236.1 ribosome maturation factor RimM -
  ACKWDP_RS17715 (ACKWDP_17715) rpsP 3638570..3638821 (-) 252 WP_000260334.1 30S ribosomal protein S16 -
  ACKWDP_RS17720 (ACKWDP_17720) pilE 3638968..3639393 (-) 426 WP_000788336.1 type IV pilin protein Machinery gene
  ACKWDP_RS17725 (ACKWDP_17725) pilY2 3639390..3639872 (-) 483 WP_001046489.1 type IV pilin protein Machinery gene
  ACKWDP_RS17730 (ACKWDP_17730) pilY1 3639883..3643737 (-) 3855 WP_000768943.1 vWA domain-containing protein Machinery gene
  ACKWDP_RS17735 (ACKWDP_17735) pilX 3643749..3644567 (-) 819 WP_000149374.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ACKWDP_RS17740 (ACKWDP_17740) pilW 3644564..3645565 (-) 1002 WP_000079197.1 PilW family protein Machinery gene
  ACKWDP_RS17745 (ACKWDP_17745) pilV 3645566..3646126 (-) 561 WP_002194578.1 type IV pilus modification protein PilV Machinery gene
  ACKWDP_RS17750 (ACKWDP_17750) fimU 3646120..3646593 (-) 474 WP_001214060.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  ACKWDP_RS17755 (ACKWDP_17755) ispH 3646763..3647713 (-) 951 WP_000407064.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACKWDP_RS17760 (ACKWDP_17760) gmk 3647836..3648465 (+) 630 WP_000015937.1 guanylate kinase -

Sequence

Protein


Download         Length: 1284 a.a.        Molecular weight: 138115.41 Da        Isoelectric Point: 7.3066

>NTDB_id=1089059 ACKWDP_RS17730 WP_000768943.1 3639883..3643737(-) (pilY1) [Acinetobacter baumannii strain A297]
MKLKLKNFKPNNLWYAVCSSSMIFTWLMTSSVVQASDLQIYASPTAGKKTIVMMLDTSGSMTNNSYGENRLAMLKNGMNA
FLASNNPVLNDTRVGLGNFSANGDSRSGQILVAAAPLGDASTLNTVGSQRYKLKQAVANLTAGGSTPSAHAYAEAAAYLM
GTTTYSETNYAIRKDSYIKRVRRSDNRTEYSYCTNYRDSQIDTANLWQPCRSNSYWSSWSTNNPGVDNATAYDTSSDWTY
YYTYYYTTFNYAVANADSGIPKSKSNDTASNPNIVVDRNATNSNAVYQSPLPAVANRQSCDGQGIYFLSDGEPNNTTNTR
SASVMSTALGSTFGADFNCSGGLSNTTADSGWACMGEFAKRLFDKTKNPAGVSIQTAFVGFGSDFSSLNSSDVKNACRLS
SRTQSDRKGDDACSPNQSTNAVAAPGYGNGGFFPTQSSQGVTDSVIAFINNLDKVPLEPLTTGAISVPYDALNPKNLQEY
GYLRAFEPNPANTYLTWRGNLKKYHVVLSGANAGAFEANSGGLVYNASGAFRTGTKDYWNSSTYTDGGKVFLGGSYANVP
LPIAGQPETRDAEGNITKYYYAVQSKIRNLFTDVSAVAADGSLTKISTSGTNLLKIPAAPPEETNPFDTVANTASYVLGK
FDPSTGQNILKAFPISLKLKILNYLGYSTDINATTLPSSLVTSNEPYLSMGGSIHSLPVQLTYNGTLDDNGNLTSAREQS
ILYGTMEGGLHIVDASSGIEQMVFVPADILNDSVASKALVVGQSDASAPAHGMDGAWVSDPAYNITTVGSGSSAVSKVTA
KQMNIYGGMRMGGSSYYGLDVLSPTSPKLLFRIGADQNDYSRMGQSWSKPVLANIRYNGSIRRVLIVGGGYDQCYEKPNI
TLTDACFTNGKAKGNAVYIIDAKTGQRLWWTSDTGSNTDNANMKHSIVSRISTLDRDADGLVDHLYFGDLGGQIFRVDLN
NNQTKTNSTYSSFGVRVVRLANLATNDSTYDGTNDYTGGNAPRFYEPPTVTIHDYGIHTFITVGIASGDRSTPLDVYPLT
GREGMTPASALSGRPVNNVYGIIDRDFVKKNLMSLTDNQLETKDITRTGLRKNPQILRTGETRVAQIFFPTTGVGKGGWY
RSLSSTSDGTEKANNSFRIKGGLKAFEEPMAITGNLIILVYDPQGTGIVAADPCLPRVVGETDRQTYCLPFGACLNSDGS
IDQNKENHSGFETQTGTNCPVGASECNKNVIGSGIRSVTFVPTEDNPPTTNSCGKLKLSGNEQGTGQWQCTSHLVPTRWY
ERYR

Nucleotide


Download         Length: 3855 bp        

>NTDB_id=1089059 ACKWDP_RS17730 WP_000768943.1 3639883..3643737(-) (pilY1) [Acinetobacter baumannii strain A297]
ATGAAACTAAAATTGAAGAATTTTAAGCCAAATAATTTATGGTATGCCGTCTGTTCAAGCTCAATGATATTCACATGGCT
GATGACAAGCTCTGTGGTGCAAGCAAGTGATTTACAAATTTATGCCTCACCTACAGCAGGTAAAAAAACAATTGTGATGA
TGTTGGATACATCAGGAAGTATGACCAACAATAGTTATGGTGAAAACCGCTTAGCCATGCTTAAAAATGGTATGAATGCT
TTTTTGGCTAGCAATAACCCTGTCTTAAATGATACGCGAGTAGGTTTAGGAAATTTCTCGGCAAATGGTGACAGCCGAAG
TGGACAAATTTTAGTGGCTGCTGCTCCTTTAGGTGATGCAAGTACTTTAAATACGGTAGGCTCACAACGTTATAAATTAA
AACAAGCTGTTGCTAATTTAACTGCGGGAGGCTCAACACCTTCAGCCCACGCTTATGCTGAAGCAGCTGCTTACTTAATG
GGGACAACCACATATTCAGAGACGAATTATGCTATTCGTAAAGATAGTTATATCAAACGTGTAAGAAGATCTGATAATAG
AACTGAATATTCATATTGTACTAATTACCGTGATTCGCAAATTGATACAGCCAACCTATGGCAACCTTGTCGTTCAAATA
GTTATTGGAGTAGCTGGTCAACAAATAATCCAGGTGTGGATAATGCTACTGCTTATGATACTTCATCTGACTGGACTTAT
TACTATACTTATTACTACACCACTTTTAATTATGCTGTAGCAAATGCAGATAGCGGTATTCCAAAATCTAAATCAAACGA
TACAGCTAGCAATCCGAATATTGTTGTAGACCGAAATGCTACTAACTCCAATGCAGTGTATCAGTCACCTTTACCTGCAG
TAGCGAATCGCCAGAGTTGTGATGGACAAGGTATTTACTTCTTATCGGATGGCGAACCGAACAATACAACAAATACACGT
TCGGCAAGTGTCATGTCGACTGCTTTAGGTAGTACTTTTGGAGCTGATTTTAATTGTTCTGGAGGTTTATCTAACACTAC
GGCAGACTCTGGTTGGGCTTGTATGGGAGAGTTTGCAAAAAGATTATTTGATAAAACAAAGAACCCTGCCGGAGTTTCTA
TTCAAACAGCATTTGTCGGTTTTGGTAGTGACTTTTCTAGTTTGAATTCTTCTGATGTAAAAAATGCCTGCCGTTTAAGT
TCGAGAACCCAGTCTGACCGAAAAGGAGATGATGCGTGTTCACCGAATCAATCTACAAATGCGGTAGCTGCACCAGGTTA
TGGAAATGGTGGTTTTTTCCCTACTCAGAGTTCACAAGGCGTAACAGACAGCGTTATCGCATTTATTAATAATTTAGATA
AAGTTCCTTTAGAACCCTTAACCACAGGTGCTATCTCAGTTCCATATGATGCATTGAATCCCAAAAATTTACAGGAATAT
GGTTATTTACGAGCTTTTGAGCCAAATCCAGCTAACACGTACTTAACATGGCGTGGAAATTTAAAGAAATATCATGTTGT
TTTATCTGGAGCGAATGCTGGTGCTTTTGAGGCCAATTCTGGCGGATTAGTCTACAATGCAAGTGGGGCTTTTAGAACTG
GAACAAAGGATTATTGGAATAGCTCTACTTATACTGACGGAGGCAAAGTCTTTTTAGGTGGTTCATATGCGAATGTGCCT
TTACCCATTGCTGGACAACCTGAAACACGTGATGCAGAAGGGAATATCACAAAATATTATTACGCAGTACAAAGTAAAAT
CCGCAACTTATTTACTGATGTTTCCGCCGTTGCAGCAGATGGTAGCTTAACGAAAATTTCAACTTCTGGAACTAATTTGC
TAAAAATTCCAGCTGCTCCACCAGAAGAAACTAATCCTTTTGATACGGTGGCTAATACAGCAAGTTATGTTTTAGGTAAA
TTTGATCCATCAACTGGACAAAATATTTTAAAAGCTTTTCCTATAAGCTTGAAATTAAAAATATTAAATTATTTAGGTTA
TTCAACAGATATTAATGCAACAACTCTGCCTTCATCTTTGGTTACATCGAATGAACCTTATTTATCGATGGGGGGAAGTA
TTCACTCTTTACCGGTTCAACTAACCTACAATGGAACCCTAGATGATAATGGGAATTTAACATCTGCCCGAGAACAATCC
ATCCTCTATGGAACTATGGAAGGCGGATTACATATTGTGGATGCTTCATCTGGTATTGAGCAAATGGTCTTTGTTCCTGC
AGATATTTTAAATGATTCAGTTGCTTCCAAAGCTTTAGTTGTTGGTCAAAGTGATGCTTCAGCTCCCGCTCATGGTATGG
ACGGAGCTTGGGTATCTGATCCAGCCTATAATATTACTACAGTGGGAAGTGGTAGCTCGGCAGTATCAAAAGTAACTGCA
AAGCAAATGAATATTTATGGCGGCATGCGTATGGGAGGAAGTAGCTACTACGGACTAGATGTATTAAGCCCTACTTCACC
GAAACTGCTTTTTAGAATAGGGGCAGACCAGAATGACTATAGCCGTATGGGTCAAAGCTGGTCTAAACCCGTACTCGCGA
ACATCCGTTATAACGGTTCTATTAGACGCGTCCTGATTGTTGGCGGTGGTTATGATCAGTGTTATGAAAAACCAAATATT
ACGTTGACTGACGCTTGCTTTACCAATGGAAAAGCAAAAGGAAATGCTGTCTATATTATTGACGCAAAAACTGGTCAGCG
TTTGTGGTGGACAAGTGATACAGGTTCTAATACTGATAACGCCAATATGAAGCATAGTATTGTTAGCCGTATTAGTACTT
TAGACCGTGATGCTGATGGCTTAGTCGACCATCTATATTTTGGAGATTTAGGCGGACAAATTTTTCGCGTAGACCTTAAT
AATAATCAGACAAAAACCAATTCGACCTATAGCAGTTTTGGTGTCAGAGTTGTGCGTTTAGCGAATTTAGCAACAAATGA
TTCAACTTATGATGGCACAAATGATTATACAGGTGGGAATGCTCCTCGTTTTTATGAGCCTCCAACAGTAACGATCCACG
ATTATGGAATTCACACTTTTATTACAGTAGGAATTGCATCAGGAGACCGTAGTACACCTTTAGATGTTTACCCACTCACA
GGTCGTGAAGGTATGACGCCTGCAAGTGCATTAAGTGGACGTCCTGTAAATAATGTATATGGAATTATTGATAGAGACTT
TGTTAAAAAGAACTTAATGTCTTTAACTGATAATCAGCTTGAAACAAAAGATATTACACGGACAGGCTTAAGAAAAAATC
CACAAATTCTAAGAACAGGTGAAACAAGAGTAGCTCAAATTTTCTTCCCAACTACAGGAGTAGGTAAAGGTGGTTGGTAT
CGCTCGCTTTCTAGTACGAGCGATGGTACAGAAAAAGCTAATAATAGTTTTCGTATTAAAGGAGGACTCAAAGCTTTTGA
GGAACCAATGGCAATTACGGGTAATTTAATTATTCTAGTTTATGACCCTCAAGGAACGGGAATTGTTGCGGCAGATCCTT
GCCTACCTCGTGTTGTGGGAGAAACAGACCGACAAACTTATTGTTTACCATTTGGAGCTTGTCTTAATTCTGATGGGTCA
ATCGATCAAAATAAAGAGAATCACAGTGGGTTTGAAACACAAACTGGTACTAATTGCCCAGTAGGGGCTTCTGAATGTAA
TAAAAACGTTATTGGCTCTGGTATTCGTAGCGTAACATTTGTACCAACGGAGGATAACCCACCTACGACTAATAGTTGTG
GGAAATTAAAGCTGTCTGGTAATGAGCAAGGGACTGGACAGTGGCAATGTACGAGTCATTTAGTTCCTACGCGTTGGTAT
GAGCGTTATCGTTAA

Domains

Predicted by InterproScan.

Neisseria PilC beta-propeller domain (T4P_PilY1)

(715-964)

von Willebrand factor type A domain (VWA_2)

(51-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilY1 Acinetobacter baumannii D1279779

90

100

0.904

Multiple sequence alignment