Detailed information NTG1088531

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACLKZ2_RS31520
• Genome accession: NZ_CP178225
• Coordinates: 6625858..6627006 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA492
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6620858..6632006

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLKZ2_RS31500 (ACLKZ2_31500)	-	6621595..6622779 (-)	1185	WP_003084564.1	cystathionine gamma-synthase	-
ACLKZ2_RS31505 (ACLKZ2_31505)	-	6622776..6624149 (-)	1374	WP_126892857.1	pyridoxal-phosphate dependent enzyme	-
ACLKZ2_RS31510 (ACLKZ2_31510)	-	6624392..6624796 (-)	405	WP_003084560.1	NINE protein	-
ACLKZ2_RS31515 (ACLKZ2_31515)	czcD	6624951..6625850 (+)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
ACLKZ2_RS31520 (ACLKZ2_31520)	pilU	6625858..6627006 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACLKZ2_RS31525 (ACLKZ2_31525)	pilT	6627184..6628218 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACLKZ2_RS31530 (ACLKZ2_31530)	-	6628433..6629125 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACLKZ2_RS31535 (ACLKZ2_31535)	proC	6629137..6629958 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACLKZ2_RS31540 (ACLKZ2_31540)	-	6629969..6630562 (+)	594	WP_003084544.1	YggT family protein	-
ACLKZ2_RS31545 (ACLKZ2_31545)	-	6630559..6630855 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1088531 ACLKZ2_RS31520 WP_003100959.1 6625858..6627006(-) (pilU) [Pseudomonas aeruginosa strain PA492]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1088531 ACLKZ2_RS31520 WP_003100959.1 6625858..6627006(-) (pilU) [Pseudomonas aeruginosa strain PA492]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382