Detailed information NTG1086557

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACLF6N_RS13580
• Genome accession: NZ_CP177279
• Coordinates: 2497727..2498344 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain KFRI-P74
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2492727..2503344

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLF6N_RS13550 (ACLF6N_13550)	rpsT	2493028..2493294 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ACLF6N_RS13555 (ACLF6N_13555)	holA	2493309..2494352 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
ACLF6N_RS13560 (ACLF6N_13560)	-	2494392..2494505 (-)	114	WP_122060479.1	hypothetical protein	-
ACLF6N_RS13565 (ACLF6N_13565)	yqzM	2494582..2494716 (+)	135	WP_003229983.1	YqzM family protein	-
ACLF6N_RS13570 (ACLF6N_13570)	comEC	2494757..2497087 (-)	2331	WP_046160607.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACLF6N_RS13575 (ACLF6N_13575)	comEB	2497091..2497660 (-)	570	WP_003229978.1	ComE operon protein 2	-
ACLF6N_RS13580 (ACLF6N_13580)	comEA	2497727..2498344 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
ACLF6N_RS13585 (ACLF6N_13585)	comER	2498428..2499249 (+)	822	WP_032726195.1	late competence protein ComER	-
ACLF6N_RS13590 (ACLF6N_13590)	yqeM	2499315..2500058 (-)	744	WP_046160609.1	class I SAM-dependent DNA methyltransferase	-
ACLF6N_RS13595 (ACLF6N_13595)	rsfS	2500055..2500411 (-)	357	WP_014480315.1	ribosome silencing factor	-
ACLF6N_RS13600 (ACLF6N_13600)	yqeK	2500429..2500989 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACLF6N_RS13605 (ACLF6N_13605)	nadD	2500979..2501548 (-)	570	WP_041333756.1	nicotinate-nucleotide adenylyltransferase	-
ACLF6N_RS13610 (ACLF6N_13610)	yhbY	2501560..2501850 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ACLF6N_RS13615 (ACLF6N_13615)	aroE	2501844..2502686 (-)	843	WP_029317883.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=1086557 ACLF6N_RS13580 WP_046160608.1 2497727..2498344(-) (comEA) [Bacillus subtilis strain KFRI-P74]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1086557 ACLF6N_RS13580 WP_046160608.1 2497727..2498344(-) (comEA) [Bacillus subtilis strain KFRI-P74]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376