Detailed information NTG1086433

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACLFAG_RS02050
• Genome accession: NZ_CP177247
• Coordinates: 439494..440642 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain WYT
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 434494..445642

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLFAG_RS02025 (ACLFAG_02025)	-	435645..435941 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACLFAG_RS02030 (ACLFAG_02030)	-	435938..436531 (-)	594	WP_003084544.1	YggT family protein	-
ACLFAG_RS02035 (ACLFAG_02035)	proC	436542..437363 (-)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
ACLFAG_RS02040 (ACLFAG_02040)	-	437375..438067 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACLFAG_RS02045 (ACLFAG_02045)	pilT	438282..439316 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACLFAG_RS02050 (ACLFAG_02050)	pilU	439494..440642 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACLFAG_RS02055 (ACLFAG_02055)	czcD	440650..441549 (-)	900	WP_073653134.1	CDF family zinc transporter CzcD	-
ACLFAG_RS02060 (ACLFAG_02060)	-	441705..442109 (+)	405	WP_003084560.1	NINE protein	-
ACLFAG_RS02065 (ACLFAG_02065)	-	442352..443725 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACLFAG_RS02070 (ACLFAG_02070)	-	443722..444906 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1086433 ACLFAG_RS02050 WP_003100959.1 439494..440642(+) (pilU) [Pseudomonas aeruginosa strain WYT]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1086433 ACLFAG_RS02050 WP_003100959.1 439494..440642(+) (pilU) [Pseudomonas aeruginosa strain WYT]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTTGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382