Detailed information NTG1086385

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACLEAH_RS02015
• Genome accession: NZ_CP177242
• Coordinates: 430187..431335 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain SM45
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 425187..436335

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLEAH_RS01990 (ACLEAH_01990)	-	426338..426634 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACLEAH_RS01995 (ACLEAH_01995)	-	426631..427224 (-)	594	WP_003084544.1	YggT family protein	-
ACLEAH_RS02000 (ACLEAH_02000)	proC	427235..428056 (-)	822	WP_023092309.1	pyrroline-5-carboxylate reductase	-
ACLEAH_RS02005 (ACLEAH_02005)	-	428068..428760 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACLEAH_RS02010 (ACLEAH_02010)	pilT	428975..430009 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACLEAH_RS02015 (ACLEAH_02015)	pilU	430187..431335 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ACLEAH_RS02020 (ACLEAH_02020)	czcD	431343..432242 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACLEAH_RS02025 (ACLEAH_02025)	-	432397..432801 (+)	405	WP_003084560.1	NINE protein	-
ACLEAH_RS02030 (ACLEAH_02030)	-	433044..434417 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
ACLEAH_RS02035 (ACLEAH_02035)	-	434414..435598 (+)	1185	WP_023092310.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1086385 ACLEAH_RS02015 WP_003110016.1 430187..431335(+) (pilU) [Pseudomonas aeruginosa strain SM45]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1086385 ACLEAH_RS02015 WP_003110016.1 430187..431335(+) (pilU) [Pseudomonas aeruginosa strain SM45]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382