Detailed information NTG1086255

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACI7Q4_RS01975
• Genome accession: NZ_CP177227
• Coordinates: 423204..424352 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain FS26
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 418204..429352

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACI7Q4_RS01950 (ACI7Q4_01950)	-	419355..419651 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACI7Q4_RS01955 (ACI7Q4_01955)	-	419648..420241 (-)	594	WP_003084544.1	YggT family protein	-
ACI7Q4_RS01960 (ACI7Q4_01960)	proC	420252..421073 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACI7Q4_RS01965 (ACI7Q4_01965)	-	421085..421777 (-)	693	WP_411956388.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACI7Q4_RS01970 (ACI7Q4_01970)	pilT	421992..423026 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACI7Q4_RS01975 (ACI7Q4_01975)	pilU	423204..424352 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACI7Q4_RS01980 (ACI7Q4_01980)	czcD	424360..425259 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACI7Q4_RS01985 (ACI7Q4_01985)	-	425413..425817 (+)	405	WP_003084560.1	NINE protein	-
ACI7Q4_RS01990 (ACI7Q4_01990)	-	426060..427433 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACI7Q4_RS01995 (ACI7Q4_01995)	-	427430..428614 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1086255 ACI7Q4_RS01975 WP_003100959.1 423204..424352(+) (pilU) [Pseudomonas aeruginosa strain FS26]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1086255 ACI7Q4_RS01975 WP_003100959.1 423204..424352(+) (pilU) [Pseudomonas aeruginosa strain FS26]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382