Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACLB90_RS05560 Genome accession   NZ_CP177163
Coordinates   1318048..1319106 (-) Length   352 a.a.
NCBI ID   WP_411851989.1    Uniprot ID   -
Organism   Stenotrophomonas sp. LGBM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1313048..1324106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB90_RS05540 (ACLB90_05540) - 1313960..1315942 (-) 1983 WP_411851985.1 type IV pilus secretin PilQ -
  ACLB90_RS05545 (ACLB90_05545) - 1315963..1316499 (-) 537 WP_411851986.1 pilus assembly protein PilP -
  ACLB90_RS05550 (ACLB90_05550) - 1316496..1317173 (-) 678 WP_411851987.1 type 4a pilus biogenesis protein PilO -
  ACLB90_RS05555 (ACLB90_05555) - 1317170..1318048 (-) 879 WP_411851988.1 PilN domain-containing protein -
  ACLB90_RS05560 (ACLB90_05560) pilM 1318048..1319106 (-) 1059 WP_411851989.1 pilus assembly protein PilM Machinery gene
  ACLB90_RS05565 (ACLB90_05565) - 1319330..1321750 (+) 2421 WP_411851990.1 penicillin-binding protein 1A -
  ACLB90_RS05570 (ACLB90_05570) - 1321864..1322511 (+) 648 WP_411851991.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37704.24 Da        Isoelectric Point: 4.4460

>NTDB_id=1085707 ACLB90_RS05560 WP_411851989.1 1318048..1319106(-) (pilM) [Stenotrophomonas sp. LGBM10]
MGLIPKSQSPLIGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVNRSGTKARHAAAA
VAGSAVITKLIPMPADLDENDMEAQVELEAVNYIPYPIEEVNLDFEVLGAIPNNPEMVQVLLAASRSENVELRQSALELG
GLTARVMDVEAFAVENAFALVASELPVASDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEVMRRYGLTYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAALAGLPEMVEEQLGVATIVANPLA
QMTLGPKVQAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1085707 ACLB90_RS05560 WP_411851989.1 1318048..1319106(-) (pilM) [Stenotrophomonas sp. LGBM10]
GTGGGGCTTATCCCAAAGAGCCAGTCGCCGCTTATTGGCGTCGACATCAGTTCGACTGCGGTAAAGCTGTTGCAGCTTTC
ACGCAGTGGCAACCGTTTTCGCGTGGAACATTACGCCGTGGAACCACTTCCGCCGAATGCGGTGGTGGAAAAGAACATCG
TCGAGGTCGAAGCGGTCGGGGAGGCCATTCGACGCGCCGTGAACCGTTCGGGGACCAAGGCCCGGCATGCCGCCGCTGCC
GTGGCAGGCTCGGCGGTGATCACCAAGCTGATCCCGATGCCGGCCGATCTGGATGAGAACGACATGGAAGCCCAGGTCGA
GCTCGAGGCGGTCAATTACATCCCGTACCCGATCGAGGAAGTGAACCTCGATTTCGAAGTGCTGGGCGCCATTCCGAACA
ACCCGGAAATGGTCCAGGTGCTGCTGGCGGCGTCGCGTTCGGAGAACGTCGAGCTGCGCCAGTCGGCGCTGGAGCTGGGC
GGGCTGACCGCGCGGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTTCGCCCTGGTCGCCAGCGAGCTGCCGGT
GGCCAGCGATGGCGTGGTGGCGCTGGTGGACATCGGTGCCACCATGACCACGCTCAATGTCCTGCGTGGCGGGCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGCTCACCGATGAAGTGATGCGCCGGTACGGCCTCACCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGCGGGTTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTATGCCGGTAGCGAGTTCAACCGTGTCGACCACATCGTGCTGGCCGGCG
GGTGCGCGGCGCTGGCCGGTCTGCCGGAGATGGTGGAAGAACAGCTGGGCGTGGCCACCATCGTGGCCAACCCGCTGGCA
CAGATGACGCTCGGCCCGAAGGTGCAGGCGCACGCGCTTGCCCAGGATGCCCCGGCGCTGATGATCGCCACGGGTCTGGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.852

100

0.509
  comM Acinetobacter nosocomialis M2

50.568

100

0.506
  comM Acinetobacter baylyi ADP1

50

100

0.5
  pilM Legionella pneumophila strain ERS1305867

47.009

99.716

0.469

Multiple sequence alignment