Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACLBNB_RS03435 Genome accession   NZ_CP177156
Coordinates   746738..747790 (+) Length   350 a.a.
NCBI ID   WP_124307497.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain YL-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 741738..752790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLBNB_RS03425 (ACLBNB_03425) - 742688..743956 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  ACLBNB_RS03430 (ACLBNB_03430) - 744086..746521 (-) 2436 WP_164485365.1 penicillin-binding protein 1A -
  ACLBNB_RS03435 (ACLBNB_03435) pilM 746738..747790 (+) 1053 WP_124307497.1 pilus assembly protein PilM Machinery gene
  ACLBNB_RS03440 (ACLBNB_03440) - 747790..748356 (+) 567 WP_081362900.1 PilN domain-containing protein -
  ACLBNB_RS03445 (ACLBNB_03445) - 748353..748970 (+) 618 WP_081362899.1 type 4a pilus biogenesis protein PilO -
  ACLBNB_RS03450 (ACLBNB_03450) - 748967..749494 (+) 528 WP_081362898.1 pilus assembly protein PilP -
  ACLBNB_RS03455 (ACLBNB_03455) pilQ 749508..751580 (+) 2073 WP_124307498.1 type IV pilus secretin PilQ Machinery gene
  ACLBNB_RS03460 (ACLBNB_03460) aroK 751585..752103 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37021.55 Da        Isoelectric Point: 4.8956

>NTDB_id=1085581 ACLBNB_RS03435 WP_124307497.1 746738..747790(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain YL-21]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLAKARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESLLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAVGVSVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSIEDAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVSGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=1085581 ACLBNB_RS03435 WP_124307497.1 746738..747790(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain YL-21]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAAGGCCCGGCCGGGAGCAAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAAAGCCTGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCTGCGATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCTTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAGGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGTCGGGGTGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGATAGAAGACGCTGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGACTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTTCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTTGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.366

100

0.551
  comM Acinetobacter nosocomialis M2

54.085

100

0.549
  comM Acinetobacter baylyi ADP1

52.113

100

0.529
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.457

Multiple sequence alignment