Detailed information NTG0108440

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: ABWW29_RS04155
• Genome accession: NZ_AP031218
• Coordinates: 836182..837993 (+)
• Length: 603 a.a.
• NCBI ID: WP_002387169.1
• Uniprot ID: A0A855UCR5
• Organism: Enterococcus faecalis strain EF01
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	825695..845053	836182..837993	within	0

Gene organization within MGE regions

Location: 825695..845053

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABWW29_RS04105 (EF01_07990)	trpS	826288..827298 (-)	1011	WP_002378619.1	tryptophan--tRNA ligase	-
ABWW29_RS04110 (EF01_08000)	-	827652..828845 (-)	1194	WP_010706472.1	site-specific integrase	-
ABWW29_RS04115 (EF01_08010)	-	828924..829127 (-)	204	WP_002607397.1	excisionase	-
ABWW29_RS04120 (EF01_08020)	-	829127..830188 (-)	1062	WP_256756141.1	replication initiation factor domain-containing protein	-
ABWW29_RS04125 (EF01_08030)	-	830731..831402 (+)	672	WP_002322214.1	helix-turn-helix transcriptional regulator	-
ABWW29_RS04130 (EF01_08040)	-	831713..832192 (+)	480	WP_002322212.1	MGMT family protein	-
ABWW29_RS04135 (EF01_08050)	dfrF	832500..832994 (+)	495	WP_002322211.1	trimethoprim-resistant dihydrofolate reductase DfrF	-
ABWW29_RS04140 (EF01_08060)	spxA	833464..833862 (+)	399	WP_002356477.1	transcriptional regulator SpxA	-
ABWW29_RS04145 (EF01_08070)	-	834204..834866 (+)	663	WP_002356478.1	adaptor protein MecA	-
ABWW29_RS04150 (EF01_08080)	-	834956..836134 (+)	1179	WP_002387170.1	competence protein CoiA family protein	-
ABWW29_RS04155 (EF01_08090)	pepF	836182..837993 (+)	1812	WP_002387169.1	oligoendopeptidase F	Regulator
ABWW29_RS04160 (EF01_08100)	-	838085..838744 (-)	660	WP_002368840.1	ClpXP adapter SpxH family protein	-
ABWW29_RS04165 (EF01_08110)	-	838808..839386 (-)	579	WP_002356483.1	CYTH domain-containing protein	-
ABWW29_RS04170 (EF01_08120)	-	839583..840257 (+)	675	WP_002356484.1	GTP pyrophosphokinase family protein	-
ABWW29_RS04175 (EF01_08130)	-	840270..841067 (+)	798	WP_002356486.1	NAD kinase	-
ABWW29_RS04180 (EF01_08140)	-	841075..841968 (+)	894	WP_002356487.1	RluA family pseudouridine synthase	-
ABWW29_RS04185 (EF01_08150)	mgtE	841999..843360 (+)	1362	WP_002362548.1	magnesium transporter	-
ABWW29_RS04190 (EF01_08160)	cutC	843478..844107 (-)	630	WP_002356490.1	copper homeostasis protein CutC	-

Sequence

Protein

• Length: 603 a.a.
• Molecular weight: 68848.16 Da
• Isoelectric Point: 4.6861

>NTDB_id=108440 ABWW29_RS04155 WP_002387169.1 836182..837993(+) (pepF) [Enterococcus faecalis strain EF01]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLIEELNQAESFKGTLGDGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide

• Length: 1812 bp

>NTDB_id=108440 ABWW29_RS04155 WP_002387169.1 836182..837993(+) (pepF) [Enterococcus faecalis strain EF01]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTGACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGATAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGTACCTTAGGGG
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGTTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAATTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

• Peptidase family M3 (Peptidase_M3) (207-587)

• Oligopeptidase F (Peptidase_M3_N) (117-186)

3D structure

• AlphaFold DB: A0A855UCR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	54.806	98.342	0.539