Detailed information NTG1083207

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACK8N4_RS02110
• Genome accession: NZ_CP176811
• Coordinates: 447398..448546 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain DG-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 442398..453546

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK8N4_RS02085 (ACK8N4_02085)	-	443549..443845 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACK8N4_RS02090 (ACK8N4_02090)	-	443842..444435 (-)	594	WP_003084544.1	YggT family protein	-
ACK8N4_RS02095 (ACK8N4_02095)	proC	444446..445267 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACK8N4_RS02100 (ACK8N4_02100)	-	445279..445971 (-)	693	WP_033972290.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACK8N4_RS02105 (ACK8N4_02105)	pilT	446186..447220 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACK8N4_RS02110 (ACK8N4_02110)	pilU	447398..448546 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACK8N4_RS02115 (ACK8N4_02115)	czcD	448554..449453 (-)	900	WP_057387713.1	CDF family zinc transporter CzcD	-
ACK8N4_RS02120 (ACK8N4_02120)	-	449607..450011 (+)	405	WP_003084560.1	NINE protein	-
ACK8N4_RS02125 (ACK8N4_02125)	-	450254..451627 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
ACK8N4_RS02130 (ACK8N4_02130)	-	451624..452808 (+)	1185	WP_033972291.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1083207 ACK8N4_RS02110 WP_003100959.1 447398..448546(+) (pilU) [Pseudomonas aeruginosa strain DG-1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1083207 ACK8N4_RS02110 WP_003100959.1 447398..448546(+) (pilU) [Pseudomonas aeruginosa strain DG-1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGTTGTCGCCGGAACAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTTTTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCCGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382