Detailed information NTG1083200

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACK3C0_RS04605
• Genome accession: NZ_CP176810
• Coordinates: 1022795..1023925 (+)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain IAS-AXF64H11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1017795..1028925

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK3C0_RS04585 (ACK3C0_04585)	-	1018119..1018943 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
ACK3C0_RS04590 (ACK3C0_04590)	-	1019560..1020387 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
ACK3C0_RS04595 (ACK3C0_04595)	-	1020772..1020984 (-)	213	WP_021358439.1	hypothetical protein	-
ACK3C0_RS04600 (ACK3C0_04600)	pilT	1021606..1022640 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
ACK3C0_RS04605 (ACK3C0_04605)	pilU	1022795..1023925 (+)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACK3C0_RS04610 (ACK3C0_04610)	-	1024594..1025562 (-)	969	WP_004084952.1	helix-turn-helix transcriptional regulator	-
ACK3C0_RS04615 (ACK3C0_04615)	-	1026345..1027154 (+)	810	WP_021358437.1	YdiY family protein	-
ACK3C0_RS04620 (ACK3C0_04620)	hemC	1027208..1028125 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
ACK3C0_RS04625 (ACK3C0_04625)	-	1028133..1028891 (-)	759	WP_004084957.1	LytR/AlgR family response regulator transcription factor	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=1083200 ACK3C0_RS04605 WP_004084950.1 1022795..1023925(+) (pilU) [Xylella fastidiosa subsp. multiplex strain IAS-AXF64H11]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=1083200 ACK3C0_RS04605 WP_004084950.1 1022795..1023925(+) (pilU) [Xylella fastidiosa subsp. multiplex strain IAS-AXF64H11]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402