Detailed information NTG1083012

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACK3B6_RS13120
• Genome accession: NZ_CP176792
• Coordinates: 2587809..2588426 (-)
• Length: 205 a.a.
• NCBI ID: WP_227597800.1
• Uniprot ID: -
• Organism: Bacillus halotolerans strain BCP32
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2582809..2593426

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK3B6_RS13090 (ACK3B6_13090)	rpsT	2583111..2583377 (+)	267	WP_202852922.1	30S ribosomal protein S20	-
ACK3B6_RS13095 (ACK3B6_13095)	holA	2583392..2584435 (-)	1044	WP_202852921.1	DNA polymerase III subunit delta	-
ACK3B6_RS13100 (ACK3B6_13100)	-	2584475..2584624 (-)	150	WP_003229985.1	hypothetical protein	-
ACK3B6_RS13105 (ACK3B6_13105)	-	2584666..2584800 (+)	135	WP_010335005.1	YqzM family protein	-
ACK3B6_RS13110 (ACK3B6_13110)	comEC	2584839..2587169 (-)	2331	WP_416043162.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACK3B6_RS13115 (ACK3B6_13115)	-	2587173..2587742 (-)	570	WP_105953575.1	ComE operon protein 2	-
ACK3B6_RS13120 (ACK3B6_13120)	comEA	2587809..2588426 (-)	618	WP_227597800.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACK3B6_RS13125 (ACK3B6_13125)	comER	2588510..2589331 (+)	822	WP_024122113.1	late competence protein ComER	-
ACK3B6_RS13130 (ACK3B6_13130)	-	2589396..2590139 (-)	744	WP_105990413.1	class I SAM-dependent DNA methyltransferase	-
ACK3B6_RS13135 (ACK3B6_13135)	rsfS	2590136..2590492 (-)	357	WP_044154484.1	ribosome silencing factor	-
ACK3B6_RS13140 (ACK3B6_13140)	yqeK	2590510..2591070 (-)	561	WP_024122116.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACK3B6_RS13145 (ACK3B6_13145)	-	2591060..2591629 (-)	570	WP_010335014.1	nicotinate-nucleotide adenylyltransferase	-
ACK3B6_RS13150 (ACK3B6_13150)	yhbY	2591640..2591930 (-)	291	WP_024122118.1	ribosome assembly RNA-binding protein YhbY	-
ACK3B6_RS13155 (ACK3B6_13155)	aroE	2591930..2592766 (-)	837	WP_416042756.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21943.84 Da
• Isoelectric Point: 5.1469

>NTDB_id=1083012 ACK3B6_RS13120 WP_227597800.1 2587809..2588426(-) (comEA) [Bacillus halotolerans strain BCP32]
MNWLNQHKTIIMSVGSAAVLIAVLCLFFFSGNNKEPVKQVVSSEKENVEVKQEATKDKAEENIVIDIKGAVKHPGVYEMH
TGDRVSQAIEKAGGIGGKADETQVNLAELLQDGTVVYIPSKGEEAAQQAAGGAVQSSKGKETLVNINTATLEDLQNISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1083012 ACK3B6_RS13120 WP_227597800.1 2587809..2588426(-) (comEA) [Bacillus halotolerans strain BCP32]
TTGAATTGGTTGAATCAACATAAAACGATAATCATGTCAGTTGGCTCTGCTGCTGTTCTCATTGCAGTTCTTTGCCTCTT
TTTTTTCAGCGGAAATAACAAAGAGCCTGTAAAACAAGTTGTATCTAGTGAAAAGGAAAATGTGGAGGTCAAGCAGGAAG
CAACAAAAGACAAAGCTGAAGAAAATATTGTAATTGATATAAAAGGCGCTGTTAAGCATCCCGGCGTTTATGAAATGCAT
ACGGGAGACAGAGTATCGCAGGCTATTGAGAAAGCAGGCGGCATTGGTGGAAAAGCAGATGAAACGCAAGTGAATTTGGC
GGAACTTTTACAGGACGGTACAGTGGTGTATATACCGAGTAAGGGAGAAGAGGCGGCACAGCAAGCGGCCGGCGGCGCTG
TACAAAGCAGTAAGGGGAAGGAGACATTGGTCAATATCAACACAGCAACATTAGAGGATTTACAAAACATTTCGGGAGTA
GGGCCTTCAAAAGCTGAAGCGATTATTGCATATCGGGAAGAGAACGGGCGTTTCCAGACAATTGAAGATATCACAAAGGT
TTCGGGAATTGGCGAAAAATCTTTTGAAAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-203)

• SLBB domain (SLBB) (64-119)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	76.699	100	0.771
comEA	Staphylococcus aureus MW2	38.288	100	0.415
comEA	Staphylococcus aureus N315	37.838	100	0.41
comEA	Latilactobacillus sakei subsp. sakei 23K	35.78	100	0.38
comEA	Streptococcus thermophilus LMD-9	36.792	100	0.38