Detailed information NTG1082328

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACK2VH_RS27520
• Genome accession: NZ_CP176684
• Coordinates: 5980276..5981424 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain GTVSS-024
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5975276..5986424

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK2VH_RS27500 (ACK2VH_27495)	-	5976014..5977198 (-)	1185	WP_003117897.1	cystathionine gamma-synthase	-
ACK2VH_RS27505 (ACK2VH_27500)	-	5977195..5978568 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACK2VH_RS27510 (ACK2VH_27505)	-	5978811..5979215 (-)	405	WP_003084560.1	NINE protein	-
ACK2VH_RS27515 (ACK2VH_27510)	czcD	5979369..5980268 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACK2VH_RS27520 (ACK2VH_27515)	pilU	5980276..5981424 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACK2VH_RS27525 (ACK2VH_27520)	pilT	5981602..5982636 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACK2VH_RS27530 (ACK2VH_27525)	-	5982851..5983543 (+)	693	WP_153655623.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACK2VH_RS27535 (ACK2VH_27530)	proC	5983555..5984376 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACK2VH_RS27540 (ACK2VH_27535)	-	5984387..5984980 (+)	594	WP_003084544.1	YggT family protein	-
ACK2VH_RS27545 (ACK2VH_27540)	-	5984977..5985273 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1082328 ACK2VH_RS27520 WP_003100959.1 5980276..5981424(-) (pilU) [Pseudomonas aeruginosa strain GTVSS-024]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1082328 ACK2VH_RS27520 WP_003100959.1 5980276..5981424(-) (pilU) [Pseudomonas aeruginosa strain GTVSS-024]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382