Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACKZ7T_RS08125 Genome accession   NZ_CP176564
Coordinates   1658400..1658897 (-) Length   165 a.a.
NCBI ID   WP_115211242.1    Uniprot ID   -
Organism   Staphylococcus aureus strain V0641     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1653400..1663897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKZ7T_RS08100 (ACKZ7T_08100) gcvPB 1653562..1655034 (-) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ACKZ7T_RS08105 (ACKZ7T_08105) gcvPA 1655027..1656373 (-) 1347 WP_250414104.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACKZ7T_RS08110 (ACKZ7T_08110) gcvT 1656393..1657484 (-) 1092 WP_409481309.1 glycine cleavage system aminomethyltransferase GcvT -
  ACKZ7T_RS08115 (ACKZ7T_08115) - 1657643..1658167 (-) 525 WP_115211241.1 shikimate kinase -
  ACKZ7T_RS08120 (ACKZ7T_08120) - 1658157..1658303 (-) 147 WP_001789879.1 hypothetical protein -
  ACKZ7T_RS08125 (ACKZ7T_08125) comGF 1658400..1658897 (-) 498 WP_115211242.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACKZ7T_RS08130 (ACKZ7T_08130) comGE 1658815..1659114 (-) 300 WP_409481310.1 competence protein ComGE Machinery gene
  ACKZ7T_RS08135 (ACKZ7T_08135) comGD 1659101..1659547 (-) 447 WP_409481311.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACKZ7T_RS08140 (ACKZ7T_08140) comGC 1659525..1659836 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACKZ7T_RS08145 (ACKZ7T_08145) comGB 1659850..1660920 (-) 1071 WP_409481312.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACKZ7T_RS08150 (ACKZ7T_08150) comGA 1660892..1661866 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACKZ7T_RS08155 (ACKZ7T_08155) - 1661919..1662542 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ACKZ7T_RS08160 (ACKZ7T_08160) - 1662539..1662868 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACKZ7T_RS08165 (ACKZ7T_08165) - 1662868..1663854 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19228.04 Da        Isoelectric Point: 10.2192

>NTDB_id=1080861 ACKZ7T_RS08125 WP_115211242.1 1658400..1658897(-) (comGF) [Staphylococcus aureus strain V0641]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLVEMLIAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNMQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1080861 ACKZ7T_RS08125 WP_115211242.1 1658400..1658897(-) (comGF) [Staphylococcus aureus strain V0641]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTGTTGAAATGTTAATAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTTATTGAAAGTAGGGAATTAACAACTGTAGATTTTGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATATGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

95.758

100

0.958

Multiple sequence alignment