Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   ACK2WG_RS12275 Genome accession   NZ_CP176536
Coordinates   2426213..2426509 (-) Length   98 a.a.
NCBI ID   WP_014114400.1    Uniprot ID   G4NQ96
Organism   Bacillus spizizenii strain 29-4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2421213..2431509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2WG_RS12230 (ACK2WG_12230) tasA 2421621..2422406 (-) 786 WP_014114390.1 biofilm matrix protein TasA -
  ACK2WG_RS12235 (ACK2WG_12235) sipW 2422470..2423042 (-) 573 WP_014114391.1 signal peptidase I SipW -
  ACK2WG_RS12240 (ACK2WG_12240) tapA 2423026..2423787 (-) 762 WP_072566543.1 amyloid fiber anchoring/assembly protein TapA -
  ACK2WG_RS12245 (ACK2WG_12245) - 2424058..2424384 (+) 327 WP_014114393.1 YqzG/YhdC family protein -
  ACK2WG_RS12250 (ACK2WG_12250) - 2424426..2424605 (-) 180 WP_003226330.1 YqzE family protein -
  ACK2WG_RS12255 (ACK2WG_12255) comGG 2424677..2425051 (-) 375 WP_072183695.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACK2WG_RS12260 (ACK2WG_12260) comGF 2425052..2425435 (-) 384 WP_041520999.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACK2WG_RS12265 (ACK2WG_12265) comGE 2425461..2425808 (-) 348 WP_242733998.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACK2WG_RS12270 (ACK2WG_12270) comGD 2425792..2426223 (-) 432 WP_014114399.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACK2WG_RS12275 (ACK2WG_12275) comGC 2426213..2426509 (-) 297 WP_014114400.1 comG operon protein ComGC Machinery gene
  ACK2WG_RS12280 (ACK2WG_12280) comGB 2426523..2427560 (-) 1038 WP_014114401.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACK2WG_RS12285 (ACK2WG_12285) comGA 2427547..2428617 (-) 1071 WP_072183690.1 competence protein ComGA Machinery gene
  ACK2WG_RS12290 (ACK2WG_12290) - 2428831..2429241 (-) 411 WP_014114403.1 CBS domain-containing protein -
  ACK2WG_RS12295 (ACK2WG_12295) - 2429304..2430251 (-) 948 WP_079287962.1 magnesium transporter CorA family protein -

Sequence

Protein


Download         Length: 98 a.a.        Molecular weight: 10844.72 Da        Isoelectric Point: 6.4730

>NTDB_id=1080787 ACK2WG_RS12275 WP_014114400.1 2426213..2426509(-) (comGC) [Bacillus spizizenii strain 29-4]
MNEKGFTLVEMLIVLFIISILLLITIPNVTKHNQTIQKKGCEGLQNMIKAQMTAFELDHEGQTPSLADLQSEGYVKKDAV
CPNGKHIIITGGEVKVEH

Nucleotide


Download         Length: 297 bp        

>NTDB_id=1080787 ACK2WG_RS12275 WP_014114400.1 2426213..2426509(-) (comGC) [Bacillus spizizenii strain 29-4]
ATGAATGAGAAAGGATTTACACTTGTTGAAATGCTAATTGTGCTCTTTATTATTTCGATCTTGCTTTTAATTACGATACC
GAACGTCACGAAACATAATCAAACCATTCAGAAAAAGGGCTGTGAAGGCCTTCAAAACATGATTAAGGCACAAATGACTG
CATTTGAGCTTGACCATGAAGGACAGACACCGAGTCTTGCTGATTTACAATCAGAGGGTTATGTGAAAAAGGATGCAGTT
TGTCCAAATGGGAAGCACATTATTATCACTGGCGGAGAAGTTAAGGTTGAACATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4NQ96

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

97.959

100

0.98
  comGC Staphylococcus aureus MW2

40.217

93.878

0.378
  comGC Staphylococcus aureus N315

40.217

93.878

0.378
  comYC Streptococcus suis isolate S10

48

76.531

0.367

Multiple sequence alignment