Detailed information NTG1080480

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACJ7VY_RS12360
• Genome accession: NZ_CP176501
• Coordinates: 2722875..2724605 (-)
• Length: 576 a.a.
• NCBI ID: WP_293764297.1
• Uniprot ID: -
• Organism: Stenotrophomonas sp. Y-13
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2724992..2736067	2722875..2724605	flank	387

Gene organization within MGE regions

Location: 2722875..2736067

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJ7VY_RS12360 (ACJ7VY_12360)	pilB	2722875..2724605 (-)	1731	WP_293764297.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACJ7VY_RS12365 (ACJ7VY_12365)	-	2724720..2724992 (+)	273	WP_409475721.1	type II toxin-antitoxin system CcdA family antitoxin	-
ACJ7VY_RS12370 (ACJ7VY_12370)	-	2724992..2725303 (+)	312	WP_313247651.1	CcdB family protein	-
ACJ7VY_RS12375 (ACJ7VY_12375)	-	2725398..2726096 (+)	699	WP_409475722.1	hypothetical protein	-
ACJ7VY_RS12380 (ACJ7VY_12380)	galE	2726118..2727092 (-)	975	WP_409475723.1	UDP-glucose 4-epimerase GalE	-
ACJ7VY_RS12385 (ACJ7VY_12385)	-	2727231..2728088 (+)	858	WP_409475724.1	glycosyltransferase family 2 protein	-
ACJ7VY_RS12390 (ACJ7VY_12390)	-	2728124..2728921 (+)	798	WP_409475725.1	class I SAM-dependent methyltransferase	-
ACJ7VY_RS12395 (ACJ7VY_12395)	-	2728945..2729859 (+)	915	WP_409475726.1	lysylphosphatidylglycerol synthase domain-containing protein	-
ACJ7VY_RS12400 (ACJ7VY_12400)	-	2729856..2730704 (+)	849	WP_409475727.1	class I SAM-dependent methyltransferase	-
ACJ7VY_RS12405 (ACJ7VY_12405)	-	2730697..2731869 (+)	1173	WP_409475728.1	glycosyltransferase	-
ACJ7VY_RS12410 (ACJ7VY_12410)	-	2731993..2733384 (-)	1392	WP_409475729.1	sulfatase-like hydrolase/transferase	-
ACJ7VY_RS12415 (ACJ7VY_12415)	-	2733804..2734166 (-)	363	WP_409475730.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Sequence

Protein

• Length: 576 a.a.
• Molecular weight: 62425.65 Da
• Isoelectric Point: 5.7611

>NTDB_id=1080480 ACJ7VY_RS12360 WP_293764297.1 2722875..2724605(-) (pilB) [Stenotrophomonas sp. Y-13]
MNTAVAANLVGITGIARRLVQDGALDETAARNAMAQAAAAKVPLAQWLAEKKLVNAAQLAAANAVEFGMPLLDASVFDAN
HNAMKLVSEELLRKHNVLPLFRRGGKLFIGTSNPTQSLDEIKFHTNLVVEPILVDEDQIRRTLDLWVASNESLAGGLGDD
EEGMGDLDVAAGDEDMGGGGDTGIDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRFRIDGLLKTVARAPVK
LRERIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPVQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCANCKKPAHLPDN
ALLAEGFTQEQIDKGIQLYEPVGCDECTEGYKGRTGIYQVMPMTDEIATIVLQGGNALDIAEAAQKIGVNDLRQSALLKA
AQGITSLTEINRVTKD

Nucleotide

• Length: 1731 bp

>NTDB_id=1080480 ACJ7VY_RS12360 WP_293764297.1 2722875..2724605(-) (pilB) [Stenotrophomonas sp. Y-13]
ATGAATACAGCCGTCGCCGCCAATCTCGTGGGCATCACCGGGATCGCCCGCCGCCTGGTGCAGGACGGCGCACTGGACGA
GACCGCGGCGCGCAACGCGATGGCGCAGGCGGCGGCGGCCAAGGTGCCGCTGGCGCAGTGGCTGGCCGAGAAGAAGCTGG
TCAACGCCGCGCAGCTGGCCGCGGCCAATGCGGTCGAGTTCGGCATGCCGCTGCTGGATGCCTCGGTGTTCGACGCCAAC
CACAACGCGATGAAGCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAGGCGTGGCGGCAAGCT
GTTCATCGGCACCAGCAACCCGACCCAGTCGCTGGACGAGATCAAGTTCCATACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTCGACCTGTGGGTGGCGAGCAACGAAAGCCTGGCCGGCGGGCTGGGCGACGAC
GAAGAGGGCATGGGCGACCTGGACGTCGCCGCCGGCGACGAGGACATGGGGGGCGGTGGCGATACCGGCATCGACGCCAA
GGGCGACGACACCCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCCGTGGCGCCTCGGACATCCACT
TCGAGCCGTACGAAGACGACTACCGGGTACGGTTCCGCATCGACGGCCTGCTCAAGACCGTGGCCCGGGCGCCGGTGAAG
CTGCGCGAGCGCATCGCCGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACGCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAACTGGGTTACGAACCGGTGCAGCAGAAGCTGTTC
CTGGACGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAGGACCCGGTTGAAATCCGCCTGCCGGGCGTGA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGTGACCTGGAGACCGCGGAGATCGCCATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACCCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCCCCGTACAACATCACCTCTT
CGGTCACCCTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTGTGCGCCAACTGCAAGAAGCCGGCGCACCTGCCAGACAAT
GCGCTGCTGGCCGAGGGGTTCACCCAGGAGCAGATCGACAAGGGCATCCAGCTCTACGAGCCGGTCGGCTGCGATGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACAGACGAGATCGCCACCATCGTGCTGCAGG
GCGGCAACGCGCTGGACATCGCCGAGGCCGCGCAGAAGATCGGGGTCAACGACCTGCGCCAGTCGGCCCTGCTCAAGGCC
GCGCAGGGGATCACCAGCCTGACCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (199-466)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (48-145)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.226	99.653	0.55
pilB	Acinetobacter baylyi ADP1	55.634	98.611	0.549
pilB	Legionella pneumophila strain ERS1305867	52.677	100	0.53
pilF	Neisseria gonorrhoeae MS11	49.648	98.611	0.49
pilB	Vibrio cholerae strain A1552	48.354	100	0.484
pilB	Vibrio parahaemolyticus RIMD 2210633	47.711	98.611	0.47
pilB	Vibrio campbellii strain DS40M4	47.007	98.611	0.464