Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACKWCM_RS16720 Genome accession   NZ_CP176416
Coordinates   3260011..3261411 (-) Length   466 a.a.
NCBI ID   WP_409362645.1    Uniprot ID   -
Organism   Bacillus safensis strain GUCCb3-7     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3255011..3266411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWCM_RS16685 (ACKWCM_16685) flgL 3255212..3256120 (-) 909 WP_024423617.1 flagellar hook-associated protein FlgL -
  ACKWCM_RS16690 (ACKWCM_16690) flgK 3256130..3257650 (-) 1521 WP_149126230.1 flagellar hook-associated protein FlgK -
  ACKWCM_RS16695 (ACKWCM_16695) - 3257666..3258148 (-) 483 WP_149126229.1 flagellar protein FlgN -
  ACKWCM_RS16700 (ACKWCM_16700) flgM 3258167..3258430 (-) 264 WP_024423620.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACKWCM_RS16705 (ACKWCM_16705) - 3258510..3258929 (-) 420 WP_024423621.1 TIGR03826 family flagellar region protein -
  ACKWCM_RS16710 (ACKWCM_16710) comFC 3258986..3259642 (-) 657 WP_244948474.1 ComF family protein Machinery gene
  ACKWCM_RS16715 (ACKWCM_16715) - 3259669..3259962 (-) 294 WP_025093967.1 late competence development ComFB family protein -
  ACKWCM_RS16720 (ACKWCM_16720) comFA 3260011..3261411 (-) 1401 WP_409362645.1 DEAD/DEAH box helicase Machinery gene
  ACKWCM_RS16725 (ACKWCM_16725) - 3261529..3262371 (-) 843 WP_024423625.1 DegV family protein -
  ACKWCM_RS16730 (ACKWCM_16730) degU 3262587..3263276 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  ACKWCM_RS16735 (ACKWCM_16735) degS 3263341..3264516 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  ACKWCM_RS16740 (ACKWCM_16740) - 3264738..3265406 (+) 669 WP_012011434.1 YigZ family protein -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 53432.42 Da        Isoelectric Point: 9.8998

>NTDB_id=1079993 ACKWCM_RS16720 WP_409362645.1 3260011..3261411(-) (comFA) [Bacillus safensis strain GUCCb3-7]
MLNMDKAMELMRQLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKANGFTCCRCGVSHKRYFAHSPCEVCQKDCVY
CRSCIMMGKAAECGFLYEWTGPQMVETCRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYA
LNQGMRVCIATPRTDVVLELEPRLRKAFQEVTITVLYGGSSQRFQITPLVITTTHQLMRYKNAFDVLIVDEVDAFPYSMD
ERLQFAVLKAMRKNRGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEQPLPVPTFHWIGHWKKKLKKNQLPPKVMNWM
HRHIANKRRILLFVPSIATMKKVTKILREQSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLG
SESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKQCGFN

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=1079993 ACKWCM_RS16720 WP_409362645.1 3260011..3261411(-) (comFA) [Bacillus safensis strain GUCCb3-7]
ATGTTGAATATGGACAAAGCAATGGAACTAATGCGGCAGCTGCATTCACGCCATCTGCTCACCGTTGAAACAAAGTGCCC
ACAGTCCAATTTAGATTGGCTGGAGGAGAAGGGCTTAGTCAATCGCTCTCCAGCCATTGAGAGGAAAGCAAATGGTTTTA
CATGCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAAGTGTGTCAAAAGGATTGTGTGTAC
TGTAGATCATGCATCATGATGGGAAAAGCAGCAGAATGTGGGTTTCTTTATGAATGGACAGGTCCACAAATGGTAGAAAC
ATGTCGAGCCGAATTAACATGGCAGGGAGAGTTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATTA
AAAACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTGCTTTTTCACGGAATCGAATATGCG
TTAAATCAAGGAATGAGGGTCTGTATTGCGACACCTAGAACAGATGTAGTGCTTGAACTCGAACCACGGCTTAGAAAAGC
ATTTCAAGAGGTGACAATTACCGTACTTTACGGAGGCAGTTCTCAAAGGTTTCAAATAACACCGCTTGTGATCACCACAA
CCCACCAGCTGATGAGATACAAAAATGCTTTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGAT
GAGCGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACAGAGGGGTTAGGATTTATTTAAGTGCGACACCCTCTAA
AAAAATGACGAGAGAGGTTTCTCGTGGAAAATTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCAACCATTACCCG
TACCGACCTTTCATTGGATCGGTCATTGGAAAAAGAAATTAAAAAAGAACCAGCTGCCCCCTAAAGTGATGAATTGGATG
CATAGACATATTGCAAATAAGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCT
TCGAGAACAATCCTTAAATGTGGAGGGGGTATCTGCCGATGATCCGGATAGGAAACAAAAGGTTCAGCACTTTAGAGATT
ACGTATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACGATTCCAAATGTTCAAGTGGGGGTACTAGGT
TCGGAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATTTCTGGGCGGGTAGGCAGACACCCGGATTATTGTACAGG
AGATGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGG
CTGCAAAAGAGTTTTCTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(325-421)

Type III restriction enzyme, res subunit (ResIII)

(117-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

52.193

97.854

0.511
  comFA/cflA Streptococcus pneumoniae Rx1

38.269

94.206

0.361
  comFA/cflA Streptococcus pneumoniae D39

38.269

94.206

0.361
  comFA/cflA Streptococcus pneumoniae R6

38.269

94.206

0.361

Multiple sequence alignment