Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   ACKWCM_RS11740 Genome accession   NZ_CP176416
Coordinates   2319828..2320121 (-) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus safensis strain GUCCb3-7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2314828..2325121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWCM_RS11690 (ACKWCM_11690) sinR 2314989..2315324 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  ACKWCM_RS11695 (ACKWCM_11695) tasA 2315392..2316192 (-) 801 WP_024422970.1 biofilm matrix protein TasA -
  ACKWCM_RS11700 (ACKWCM_11700) sipW 2316247..2316819 (-) 573 WP_024422971.1 signal peptidase I SipW -
  ACKWCM_RS11705 (ACKWCM_11705) tapA 2316872..2317399 (-) 528 WP_098677430.1 amyloid fiber anchoring/assembly protein TapA -
  ACKWCM_RS11710 (ACKWCM_11710) - 2317699..2317977 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  ACKWCM_RS11715 (ACKWCM_11715) - 2318011..2318205 (-) 195 WP_056767455.1 YqzE family protein -
  ACKWCM_RS11720 (ACKWCM_11720) - 2318272..2318655 (-) 384 WP_197172668.1 competence type IV pilus minor pilin ComGG -
  ACKWCM_RS11725 (ACKWCM_11725) comGF 2318652..2319179 (-) 528 WP_311164128.1 competence type IV pilus minor pilin ComGF -
  ACKWCM_RS11730 (ACKWCM_11730) comGE 2319091..2319405 (-) 315 WP_087978326.1 competence type IV pilus minor pilin ComGE -
  ACKWCM_RS11735 (ACKWCM_11735) comGD 2319389..2319835 (-) 447 WP_197172667.1 competence type IV pilus minor pilin ComGD -
  ACKWCM_RS11740 (ACKWCM_11740) comGC 2319828..2320121 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  ACKWCM_RS11745 (ACKWCM_11745) comGB 2320138..2321178 (-) 1041 WP_197172666.1 competence type IV pilus assembly protein ComGB -
  ACKWCM_RS11750 (ACKWCM_11750) comGA 2321159..2322229 (-) 1071 WP_241743547.1 competence type IV pilus ATPase ComGA Machinery gene
  ACKWCM_RS11755 (ACKWCM_11755) - 2322406..2323281 (-) 876 WP_024422979.1 STAS domain-containing protein -
  ACKWCM_RS11765 (ACKWCM_11765) - 2323505..2323885 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACKWCM_RS11770 (ACKWCM_11770) - 2324098..2324340 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  ACKWCM_RS11775 (ACKWCM_11775) - 2324388..2325026 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=1079975 ACKWCM_RS11740 WP_024422977.1 2319828..2320121(-) (comGC) [Bacillus safensis strain GUCCb3-7]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=1079975 ACKWCM_RS11740 WP_024422977.1 2319828..2320121(-) (comGC) [Bacillus safensis strain GUCCb3-7]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATAGTCATGCTCGTAATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCGATTCAGGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTCAGCACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAGGGATTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67

  comGC Staphylococcus aureus MW2

42.222

92.784

0.392

  comGC Staphylococcus aureus N315

42.222

92.784

0.392


Multiple sequence alignment