Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACKWCM_RS11750 Genome accession   NZ_CP176416
Coordinates   2321159..2322229 (-) Length   356 a.a.
NCBI ID   WP_241743547.1    Uniprot ID   -
Organism   Bacillus safensis strain GUCCb3-7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2316159..2327229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWCM_RS11700 (ACKWCM_11700) sipW 2316247..2316819 (-) 573 WP_024422971.1 signal peptidase I SipW -
  ACKWCM_RS11705 (ACKWCM_11705) tapA 2316872..2317399 (-) 528 WP_098677430.1 amyloid fiber anchoring/assembly protein TapA -
  ACKWCM_RS11710 (ACKWCM_11710) - 2317699..2317977 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  ACKWCM_RS11715 (ACKWCM_11715) - 2318011..2318205 (-) 195 WP_056767455.1 YqzE family protein -
  ACKWCM_RS11720 (ACKWCM_11720) - 2318272..2318655 (-) 384 WP_197172668.1 competence type IV pilus minor pilin ComGG -
  ACKWCM_RS11725 (ACKWCM_11725) comGF 2318652..2319179 (-) 528 WP_311164128.1 competence type IV pilus minor pilin ComGF -
  ACKWCM_RS11730 (ACKWCM_11730) comGE 2319091..2319405 (-) 315 WP_087978326.1 competence type IV pilus minor pilin ComGE -
  ACKWCM_RS11735 (ACKWCM_11735) comGD 2319389..2319835 (-) 447 WP_197172667.1 competence type IV pilus minor pilin ComGD -
  ACKWCM_RS11740 (ACKWCM_11740) comGC 2319828..2320121 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  ACKWCM_RS11745 (ACKWCM_11745) comGB 2320138..2321178 (-) 1041 WP_197172666.1 competence type IV pilus assembly protein ComGB -
  ACKWCM_RS11750 (ACKWCM_11750) comGA 2321159..2322229 (-) 1071 WP_241743547.1 competence type IV pilus ATPase ComGA Machinery gene
  ACKWCM_RS11755 (ACKWCM_11755) - 2322406..2323281 (-) 876 WP_024422979.1 STAS domain-containing protein -
  ACKWCM_RS11765 (ACKWCM_11765) - 2323505..2323885 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACKWCM_RS11770 (ACKWCM_11770) - 2324098..2324340 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  ACKWCM_RS11775 (ACKWCM_11775) - 2324388..2325026 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -
  ACKWCM_RS11780 (ACKWCM_11780) - 2325184..2325357 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  ACKWCM_RS11785 (ACKWCM_11785) - 2325382..2325696 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  ACKWCM_RS11790 (ACKWCM_11790) - 2325773..2327026 (-) 1254 WP_041117981.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40385.84 Da        Isoelectric Point: 9.5360

>NTDB_id=1079976 ACKWCM_RS11750 WP_241743547.1 2321159..2322229(-) (comGA) [Bacillus safensis strain GUCCb3-7]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQSGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1079976 ACKWCM_RS11750 WP_241743547.1 2321159..2322229(-) (comGA) [Bacillus safensis strain GUCCb3-7]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAGTGGCTCACTAGCT
GTGATGCTAAGAAAAGGTCAAGTATTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATATTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTTGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment