Detailed information
Bioinformatically predicted
Overview
| Name | pilU | Type | Machinery gene |
| Locus tag | ACJ229_RS07605 | Genome accession | NZ_CP175656 |
| Coordinates | 1469843..1470961 (-) | Length | 372 a.a. |
| NCBI ID | WP_000347039.1 | Uniprot ID | A0A9P2XLL8 |
| Organism | Acinetobacter baumannii strain UMB001 | ||
| Function | mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 1428498..1495468 | 1469843..1470961 | within | 0 |
Gene organization within MGE regions
Location: 1428498..1495468
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACJ229_RS07400 (ACJ229_07405) | - | 1429133..1430758 (-) | 1626 | WP_000834616.1 | NAD+ synthase | - |
| ACJ229_RS07405 (ACJ229_07410) | - | 1431143..1431703 (-) | 561 | WP_001048150.1 | hypothetical protein | - |
| ACJ229_RS07410 (ACJ229_07415) | - | 1431794..1432654 (-) | 861 | WP_000838098.1 | pirin family protein | - |
| ACJ229_RS07415 (ACJ229_07420) | hemJ | 1432825..1433277 (-) | 453 | WP_000339889.1 | protoporphyrinogen oxidase HemJ | - |
| ACJ229_RS07420 (ACJ229_07425) | - | 1433296..1434525 (-) | 1230 | WP_000832710.1 | beta-ketoacyl-ACP synthase II | - |
| ACJ229_RS07425 (ACJ229_07430) | - | 1434822..1436147 (+) | 1326 | WP_000160011.1 | EcsC family protein | - |
| ACJ229_RS07430 (ACJ229_07435) | rpsL | 1436348..1436722 (+) | 375 | WP_000246374.1 | 30S ribosomal protein S12 | - |
| ACJ229_RS07435 (ACJ229_07440) | rpsG | 1436882..1437352 (+) | 471 | WP_001138055.1 | 30S ribosomal protein S7 | - |
| ACJ229_RS07440 (ACJ229_07445) | fusA | 1437531..1439669 (+) | 2139 | WP_000113824.1 | elongation factor G | - |
| ACJ229_RS07445 (ACJ229_07450) | tuf | 1439764..1440954 (+) | 1191 | WP_001029610.1 | elongation factor Tu | - |
| ACJ229_RS07450 (ACJ229_07455) | - | 1441158..1442039 (+) | 882 | WP_000992305.1 | metal-dependent hydrolase | - |
| ACJ229_RS07455 (ACJ229_07460) | - | 1442276..1443151 (+) | 876 | WP_000992291.1 | metal-dependent hydrolase | - |
| ACJ229_RS07460 (ACJ229_07465) | rimI | 1443260..1443715 (+) | 456 | WP_000621543.1 | ribosomal protein S18-alanine N-acetyltransferase | - |
| ACJ229_RS07465 (ACJ229_07470) | - | 1443760..1444575 (-) | 816 | WP_000844343.1 | arginyltransferase | - |
| ACJ229_RS07470 (ACJ229_07475) | aat | 1444594..1445325 (-) | 732 | WP_000854785.1 | leucyl/phenylalanyl-tRNA--protein transferase | - |
| ACJ229_RS07475 (ACJ229_07480) | trxB | 1445445..1446392 (-) | 948 | WP_001276144.1 | thioredoxin-disulfide reductase | - |
| ACJ229_RS07480 (ACJ229_07485) | - | 1446662..1449694 (+) | 3033 | WP_000127823.1 | DNA translocase FtsK | - |
| ACJ229_RS07485 (ACJ229_07490) | rhtC | 1449844..1450464 (+) | 621 | WP_000959259.1 | threonine export protein RhtC | - |
| ACJ229_RS07490 (ACJ229_07495) | - | 1450520..1451050 (-) | 531 | WP_031969348.1 | tyrosyl-tRNA synthetase | - |
| ACJ229_RS07495 (ACJ229_07500) | - | 1451193..1452839 (-) | 1647 | WP_001094415.1 | IS66-like element ISAba17 family transposase | - |
| ACJ229_RS07500 (ACJ229_07505) | tnpB | 1452914..1453249 (-) | 336 | WP_000618090.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| ACJ229_RS07505 (ACJ229_07510) | tnpA | 1453246..1453674 (-) | 429 | WP_000364665.1 | IS66-like element accessory protein TnpA | - |
| ACJ229_RS07510 (ACJ229_07515) | - | 1453756..1454001 (-) | 246 | Protein_1334 | tyrosyl-tRNA synthetase | - |
| ACJ229_RS07515 (ACJ229_07520) | - | 1454149..1454436 (-) | 288 | WP_001218560.1 | PA4642 family protein | - |
| ACJ229_RS07520 (ACJ229_07525) | minE | 1454520..1454792 (-) | 273 | WP_000896934.1 | cell division topological specificity factor MinE | - |
| ACJ229_RS07525 (ACJ229_07530) | minD | 1454795..1455607 (-) | 813 | WP_001074362.1 | septum site-determining protein MinD | - |
| ACJ229_RS07530 (ACJ229_07535) | minC | 1455678..1456400 (-) | 723 | WP_000763677.1 | septum site-determining protein MinC | - |
| ACJ229_RS07535 (ACJ229_07540) | - | 1456522..1457568 (-) | 1047 | WP_001181639.1 | hypothetical protein | - |
| ACJ229_RS07540 (ACJ229_07545) | - | 1457585..1458511 (-) | 927 | WP_000100965.1 | acyltransferase | - |
| ACJ229_RS07545 (ACJ229_07550) | - | 1458756..1459409 (+) | 654 | WP_001202415.1 | OmpA family protein | - |
| ACJ229_RS07550 (ACJ229_07555) | rep | 1459603..1461642 (+) | 2040 | WP_000093035.1 | DNA helicase Rep | - |
| ACJ229_RS07555 (ACJ229_07560) | dut | 1461667..1462119 (+) | 453 | WP_000868152.1 | dUTP diphosphatase | - |
| ACJ229_RS07560 (ACJ229_07565) | - | 1462319..1463737 (+) | 1419 | WP_001102845.1 | phosphomannomutase/phosphoglucomutase | - |
| ACJ229_RS07565 (ACJ229_07570) | argB | 1463752..1464660 (+) | 909 | WP_001135419.1 | acetylglutamate kinase | - |
| ACJ229_RS07570 (ACJ229_07575) | - | 1464781..1465563 (+) | 783 | WP_000890283.1 | GNAT family N-acetyltransferase | - |
| ACJ229_RS07575 (ACJ229_07580) | - | 1465672..1466520 (+) | 849 | WP_000336548.1 | class II glutamine amidotransferase | - |
| ACJ229_RS07580 (ACJ229_07585) | - | 1466773..1467228 (+) | 456 | WP_000782976.1 | bacteriohemerythrin | - |
| ACJ229_RS07585 (ACJ229_07590) | - | 1467381..1468448 (+) | 1068 | WP_002019572.1 | hypothetical protein | - |
| ACJ229_RS07590 (ACJ229_07595) | - | 1468448..1468771 (+) | 324 | WP_000442587.1 | RnfH family protein | - |
| ACJ229_RS07595 (ACJ229_07600) | - | 1468826..1469224 (-) | 399 | WP_001170994.1 | outer membrane protein assembly factor BamE | - |
| ACJ229_RS07600 (ACJ229_07605) | fur | 1469336..1469773 (+) | 438 | WP_001122847.1 | ferric iron uptake transcriptional regulator | - |
| ACJ229_RS07605 (ACJ229_07610) | pilU | 1469843..1470961 (-) | 1119 | WP_000347039.1 | PilT/PilU family type 4a pilus ATPase | Machinery gene |
| ACJ229_RS07610 (ACJ229_07615) | pilT | 1470989..1472026 (-) | 1038 | WP_000355489.1 | type IV pilus twitching motility protein PilT | Machinery gene |
| ACJ229_RS07615 (ACJ229_07620) | - | 1472153..1472845 (+) | 693 | WP_001108512.1 | YggS family pyridoxal phosphate-dependent enzyme | - |
| ACJ229_RS07620 (ACJ229_07625) | - | 1473047..1476643 (-) | 3597 | WP_000698785.1 | AAA family ATPase | - |
| ACJ229_RS07625 (ACJ229_07630) | - | 1476653..1477921 (-) | 1269 | WP_000116701.1 | exonuclease SbcCD subunit D | - |
| ACJ229_RS07630 (ACJ229_07635) | - | 1478085..1478558 (+) | 474 | WP_000974358.1 | OsmC family protein | - |
| ACJ229_RS07635 (ACJ229_07640) | - | 1478626..1479003 (-) | 378 | WP_001059438.1 | VOC family protein | - |
| ACJ229_RS07640 (ACJ229_07645) | - | 1479383..1479835 (+) | 453 | WP_000164153.1 | ABZJ_00895 family protein | - |
| ACJ229_RS07645 (ACJ229_07650) | - | 1479925..1480830 (+) | 906 | WP_001039356.1 | TIGR01777 family oxidoreductase | - |
| ACJ229_RS07650 (ACJ229_07655) | - | 1480822..1481937 (-) | 1116 | WP_000546701.1 | FAD-binding oxidoreductase | - |
| ACJ229_RS07655 (ACJ229_07660) | hemB | 1481963..1482976 (-) | 1014 | WP_000222569.1 | porphobilinogen synthase | - |
| ACJ229_RS07660 (ACJ229_07665) | - | 1483119..1483616 (+) | 498 | WP_002019577.1 | thioesterase family protein | - |
| ACJ229_RS07665 (ACJ229_07670) | - | 1483886..1485037 (+) | 1152 | WP_000128703.1 | EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit | - |
| ACJ229_RS07670 (ACJ229_07675) | - | 1485044..1486567 (+) | 1524 | WP_000857095.1 | DHA2 family efflux MFS transporter permease subunit | - |
| ACJ229_RS07675 (ACJ229_07680) | ggt | 1486870..1488855 (+) | 1986 | WP_001042923.1 | gamma-glutamyltransferase | - |
| ACJ229_RS07685 (ACJ229_07690) | - | 1489557..1490786 (+) | 1230 | WP_000831316.1 | tyrosine-type recombinase/integrase | - |
| ACJ229_RS07690 (ACJ229_07695) | - | 1490934..1491701 (+) | 768 | WP_000578616.1 | hypothetical protein | - |
| ACJ229_RS07695 (ACJ229_07700) | - | 1491911..1492126 (+) | 216 | WP_000153154.1 | hypothetical protein | - |
| ACJ229_RS07700 (ACJ229_07705) | - | 1492129..1492338 (+) | 210 | WP_001016479.1 | helix-turn-helix domain-containing protein | - |
| ACJ229_RS07705 (ACJ229_07710) | - | 1492335..1492889 (+) | 555 | WP_001988245.1 | BRO family protein | - |
| ACJ229_RS07710 (ACJ229_07715) | - | 1492882..1493250 (+) | 369 | WP_001046481.1 | hypothetical protein | - |
| ACJ229_RS07715 (ACJ229_07720) | - | 1493260..1493565 (+) | 306 | WP_000787149.1 | hypothetical protein | - |
| ACJ229_RS07720 (ACJ229_07725) | - | 1493690..1493968 (+) | 279 | WP_001064707.1 | hypothetical protein | - |
Sequence
Protein
Download Length: 372 a.a. Molecular weight: 41933.13 Da Isoelectric Point: 6.6944
>NTDB_id=1078550 ACJ229_RS07605 WP_000347039.1 1469843..1470961(-) (pilU) [Acinetobacter baumannii strain UMB001]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ
Nucleotide
Download Length: 1119 bp
>NTDB_id=1078550 ACJ229_RS07605 WP_000347039.1 1469843..1470961(-) (pilU) [Acinetobacter baumannii strain UMB001]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilU | Acinetobacter baylyi ADP1 |
75.926 |
100 |
0.772 |
| pilU | Pseudomonas stutzeri DSM 10701 |
69.565 |
98.925 |
0.688 |
| pilU | Vibrio cholerae strain A1552 |
53.243 |
99.462 |
0.53 |
| pilT | Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 |
42.486 |
93.011 |
0.395 |