Detailed information NTG1078529

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACJ229_RS01635
• Genome accession: NZ_CP175656
• Coordinates: 215255..216313 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain UMB001
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 210255..221313

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJ229_RS01610 (ACJ229_01615)	aroK	210525..211094 (-)	570	WP_000643712.1	shikimate kinase AroK	-
ACJ229_RS01615 (ACJ229_01620)	pilQ	211106..213271 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
ACJ229_RS01620 (ACJ229_01625)	pilP	213339..213866 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
ACJ229_RS01625 (ACJ229_01630)	pilO	213877..214617 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACJ229_RS01630 (ACJ229_01635)	pilN	214614..215255 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
ACJ229_RS01635 (ACJ229_01640)	pilM	215255..216313 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
ACJ229_RS01640 (ACJ229_01645)	ponA	216476..219031 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
ACJ229_RS01645 (ACJ229_01650)	-	219049..219873 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
ACJ229_RS01650 (ACJ229_01655)	-	219876..220208 (-)	333	WP_000169594.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=1078529 ACJ229_RS01635 WP_002014066.1 215255..216313(-) (pilM) [Acinetobacter baumannii strain UMB001]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=1078529 ACJ229_RS01635 WP_002014066.1 215255..216313(-) (pilM) [Acinetobacter baumannii strain UMB001]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423