Detailed information NTG1078363

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACJ228_RS13885
• Genome accession: NZ_CP175642
• Coordinates: 2908289..2909407 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain G7
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2903289..2914407

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJ228_RS13855 (ACJ228_13855)	-	2904118..2904966 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
ACJ228_RS13860 (ACJ228_13860)	-	2905219..2905674 (+)	456	WP_000782976.1	bacteriohemerythrin	-
ACJ228_RS13865 (ACJ228_13865)	-	2905827..2906894 (+)	1068	WP_000092403.1	hypothetical protein	-
ACJ228_RS13870 (ACJ228_13870)	-	2906894..2907217 (+)	324	WP_000442585.1	RnfH family protein	-
ACJ228_RS13875 (ACJ228_13875)	-	2907272..2907670 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACJ228_RS13880 (ACJ228_13880)	fur	2907782..2908219 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACJ228_RS13885 (ACJ228_13885)	pilU	2908289..2909407 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACJ228_RS13890 (ACJ228_13890)	pilT	2909435..2910472 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
ACJ228_RS13895 (ACJ228_13895)	-	2910599..2911291 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=1078363 ACJ228_RS13885 WP_000347036.1 2908289..2909407(-) (pilU) [Acinetobacter baumannii strain G7]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1078363 ACJ228_RS13885 WP_000347036.1 2908289..2909407(-) (pilU) [Acinetobacter baumannii strain G7]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392