Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACKFA7_RS11645 Genome accession   NZ_CP175553
Coordinates   2467939..2469009 (-) Length   356 a.a.
NCBI ID   WP_038459186.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain 4-9-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2462939..2474009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKFA7_RS11595 (ACKFA7_11595) sipW 2462998..2463582 (-) 585 WP_012117977.1 signal peptidase I SipW -
  ACKFA7_RS11600 (ACKFA7_11600) tapA 2463554..2464225 (-) 672 WP_038459173.1 amyloid fiber anchoring/assembly protein TapA -
  ACKFA7_RS11605 (ACKFA7_11605) - 2464484..2464813 (+) 330 WP_038459175.1 DUF3889 domain-containing protein -
  ACKFA7_RS11610 (ACKFA7_11610) - 2464854..2465033 (-) 180 WP_022552966.1 YqzE family protein -
  ACKFA7_RS11615 (ACKFA7_11615) comGG 2465090..2465467 (-) 378 WP_038459177.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACKFA7_RS11620 (ACKFA7_11620) comGF 2465468..2465968 (-) 501 WP_228842201.1 competence type IV pilus minor pilin ComGF -
  ACKFA7_RS11625 (ACKFA7_11625) comGE 2465877..2466191 (-) 315 WP_038459179.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACKFA7_RS11630 (ACKFA7_11630) comGD 2466175..2466612 (-) 438 WP_038459181.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACKFA7_RS11635 (ACKFA7_11635) comGC 2466602..2466910 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  ACKFA7_RS11640 (ACKFA7_11640) comGB 2466915..2467952 (-) 1038 WP_038459183.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACKFA7_RS11645 (ACKFA7_11645) comGA 2467939..2469009 (-) 1071 WP_038459186.1 competence type IV pilus ATPase ComGA Machinery gene
  ACKFA7_RS11650 (ACKFA7_11650) - 2469206..2470156 (-) 951 WP_032874012.1 magnesium transporter CorA family protein -
  ACKFA7_RS11655 (ACKFA7_11655) - 2470302..2471603 (+) 1302 WP_038459188.1 hemolysin family protein -
  ACKFA7_RS11660 (ACKFA7_11660) - 2471641..2472471 (-) 831 WP_038459190.1 STAS domain-containing protein -
  ACKFA7_RS11670 (ACKFA7_11670) - 2472677..2473057 (-) 381 WP_038459192.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACKFA7_RS11675 (ACKFA7_11675) - 2473294..2473539 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40528.87 Da        Isoelectric Point: 9.5864

>NTDB_id=1078152 ACKFA7_RS11645 WP_038459186.1 2467939..2469009(-) (comGA) [Bacillus amyloliquefaciens strain 4-9-2]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEQNCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1078152 ACKFA7_RS11645 WP_038459186.1 2467939..2469009(-) (comGA) [Bacillus amyloliquefaciens strain 4-9-2]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCATATACGGCAAGGGCTTCAGATATACACATTGTACC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGGAAGCCGCAAAACGGGTCATTGTCT
TTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGCAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAATTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.607

98.596

0.371

Multiple sequence alignment