Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE   Type   Machinery gene
Locus tag   ACKFA7_RS11625 Genome accession   NZ_CP175553
Coordinates   2465877..2466191 (-) Length   104 a.a.
NCBI ID   WP_038459179.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain 4-9-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2460877..2471191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKFA7_RS11580 (ACKFA7_11580) sinI 2461558..2461731 (+) 174 WP_007612543.1 anti-repressor SinI Regulator
  ACKFA7_RS11585 (ACKFA7_11585) sinR 2461765..2462100 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  ACKFA7_RS11590 (ACKFA7_11590) tasA 2462148..2462933 (-) 786 WP_003153102.1 biofilm matrix protein TasA -
  ACKFA7_RS11595 (ACKFA7_11595) sipW 2462998..2463582 (-) 585 WP_012117977.1 signal peptidase I SipW -
  ACKFA7_RS11600 (ACKFA7_11600) tapA 2463554..2464225 (-) 672 WP_038459173.1 amyloid fiber anchoring/assembly protein TapA -
  ACKFA7_RS11605 (ACKFA7_11605) - 2464484..2464813 (+) 330 WP_038459175.1 DUF3889 domain-containing protein -
  ACKFA7_RS11610 (ACKFA7_11610) - 2464854..2465033 (-) 180 WP_022552966.1 YqzE family protein -
  ACKFA7_RS11615 (ACKFA7_11615) comGG 2465090..2465467 (-) 378 WP_038459177.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACKFA7_RS11620 (ACKFA7_11620) comGF 2465468..2465968 (-) 501 WP_228842201.1 competence type IV pilus minor pilin ComGF -
  ACKFA7_RS11625 (ACKFA7_11625) comGE 2465877..2466191 (-) 315 WP_038459179.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACKFA7_RS11630 (ACKFA7_11630) comGD 2466175..2466612 (-) 438 WP_038459181.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACKFA7_RS11635 (ACKFA7_11635) comGC 2466602..2466910 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  ACKFA7_RS11640 (ACKFA7_11640) comGB 2466915..2467952 (-) 1038 WP_038459183.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACKFA7_RS11645 (ACKFA7_11645) comGA 2467939..2469009 (-) 1071 WP_038459186.1 competence type IV pilus ATPase ComGA Machinery gene
  ACKFA7_RS11650 (ACKFA7_11650) - 2469206..2470156 (-) 951 WP_032874012.1 magnesium transporter CorA family protein -

Sequence

Protein


Download         Length: 104 a.a.        Molecular weight: 11932.91 Da        Isoelectric Point: 5.8321

>NTDB_id=1078148 ACKFA7_RS11625 WP_038459179.1 2465877..2466191(-) (comGE) [Bacillus amyloliquefaciens strain 4-9-2]
MLNGNKGFSTIETLSAMAIWLFLMTSIIPVWTDMLTDGLKIEDRQEAYQLLQKHISTYMMTGKKPPSPGVKWKEDGEYYK
VCAADRSEKEMCLSILKTDWLYAS

Nucleotide


Download         Length: 315 bp        

>NTDB_id=1078148 ACKFA7_RS11625 WP_038459179.1 2465877..2466191(-) (comGE) [Bacillus amyloliquefaciens strain 4-9-2]
ATGCTAAACGGAAATAAGGGGTTCTCTACTATTGAAACACTATCAGCAATGGCCATTTGGCTGTTCCTTATGACGTCTAT
CATTCCGGTCTGGACGGACATGCTGACAGATGGTCTGAAAATAGAAGATCGCCAGGAAGCGTACCAGCTTCTTCAGAAAC
ATATCAGCACTTATATGATGACCGGAAAAAAGCCGCCGTCTCCCGGTGTGAAGTGGAAGGAGGATGGTGAGTATTACAAA
GTGTGTGCGGCTGACCGCAGCGAAAAAGAAATGTGCCTCAGTATTCTCAAAACAGACTGGCTTTACGCTTCTTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE Bacillus subtilis subsp. subtilis str. 168

43.478

100

0.481

Multiple sequence alignment