Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   ECOLIN_RS10360 Genome accession   NZ_CP007799
Coordinates   2028337..2029347 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli Nissle 1917     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2023337..2034347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECOLIN_RS10340 (ECOLIN_09970) mepM 2024304..2025626 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  ECOLIN_RS10345 (ECOLIN_09975) znuA 2025642..2026574 (-) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  ECOLIN_RS10350 (ECOLIN_09980) znuC 2026653..2027408 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  ECOLIN_RS10355 (ECOLIN_09985) znuB 2027405..2028190 (+) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  ECOLIN_RS10360 (ECOLIN_09990) ruvB 2028337..2029347 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  ECOLIN_RS10365 (ECOLIN_09995) ruvA 2029356..2029967 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  ECOLIN_RS30690 yobI 2030106..2030171 (-) 66 WP_072123646.1 stress response small protein YobI -
  ECOLIN_RS10370 (ECOLIN_10000) yebB 2030243..2030845 (+) 603 WP_001024947.1 YebB family permuted papain-like enzyme -
  ECOLIN_RS10375 (ECOLIN_10005) ruvC 2030847..2031368 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  ECOLIN_RS10380 (ECOLIN_10010) yebC 2031403..2032143 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  ECOLIN_RS10385 (ECOLIN_10015) nudB 2032172..2032624 (-) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=107789 ECOLIN_RS10360 WP_000568522.1 2028337..2029347(-) (ruvB) [Escherichia coli Nissle 1917]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=107789 ECOLIN_RS10360 WP_000568522.1 2028337..2029347(-) (ruvB) [Escherichia coli Nissle 1917]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCTGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment