Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGD   Type   Machinery gene
Locus tag   ACJGE4_RS12200 Genome accession   NZ_CP174519
Coordinates   2521310..2521747 (-) Length   145 a.a.
NCBI ID   WP_043020787.1    Uniprot ID   -
Organism   Bacillus velezensis strain JD-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2516310..2526747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJGE4_RS12150 (ACJGE4_12150) sinI 2516694..2516867 (+) 174 WP_003153105.1 anti-repressor SinI Regulator
  ACJGE4_RS12155 (ACJGE4_12155) sinR 2516901..2517236 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  ACJGE4_RS12160 (ACJGE4_12160) tasA 2517284..2518069 (-) 786 WP_407966252.1 biofilm matrix protein TasA -
  ACJGE4_RS12165 (ACJGE4_12165) sipW 2518134..2518718 (-) 585 WP_015240205.1 signal peptidase I SipW -
  ACJGE4_RS12170 (ACJGE4_12170) tapA 2518690..2519361 (-) 672 WP_020954230.1 amyloid fiber anchoring/assembly protein TapA -
  ACJGE4_RS12175 (ACJGE4_12175) - 2519620..2519949 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  ACJGE4_RS12180 (ACJGE4_12180) - 2519989..2520168 (-) 180 WP_003153093.1 YqzE family protein -
  ACJGE4_RS12185 (ACJGE4_12185) comGG 2520225..2520602 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACJGE4_RS12190 (ACJGE4_12190) comGF 2520603..2521103 (-) 501 WP_257899725.1 competence type IV pilus minor pilin ComGF -
  ACJGE4_RS12195 (ACJGE4_12195) comGE 2521012..2521326 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  ACJGE4_RS12200 (ACJGE4_12200) comGD 2521310..2521747 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACJGE4_RS12205 (ACJGE4_12205) comGC 2521737..2522003 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  ACJGE4_RS12210 (ACJGE4_12210) comGB 2522050..2523087 (-) 1038 WP_094031834.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACJGE4_RS12215 (ACJGE4_12215) comGA 2523074..2524144 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  ACJGE4_RS12220 (ACJGE4_12220) - 2524338..2525288 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  ACJGE4_RS12225 (ACJGE4_12225) - 2525434..2526735 (+) 1302 WP_021494315.1 hemolysin family protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16271.77 Da        Isoelectric Point: 10.2475

>NTDB_id=1077727 ACJGE4_RS12200 WP_043020787.1 2521310..2521747(-) (comGD) [Bacillus velezensis strain JD-3]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPAYTHLAVRQKTELLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1077727 ACJGE4_RS12200 WP_043020787.1 2521310..2521747(-) (comGD) [Bacillus velezensis strain JD-3]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGCCTACACCCACCTTGCCGTGCGGCAAAAAACAGAACTGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAGGATTGTCGAGCGTTCTTTTGACTCGCTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCAATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTTCATGCTAAACGGAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

56.164

100

0.566


Multiple sequence alignment