Detailed information NTG1077500

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACJSOD_RS09585
• Genome accession: NZ_CP174508
• Coordinates: 2068772..2069920 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain KS1-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2063772..2074920

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJSOD_RS09560 (ACJSOD_09560)	-	2064923..2065219 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACJSOD_RS09565 (ACJSOD_09565)	-	2065216..2065809 (-)	594	WP_003084544.1	YggT family protein	-
ACJSOD_RS09570 (ACJSOD_09570)	proC	2065820..2066641 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACJSOD_RS09575 (ACJSOD_09575)	-	2066653..2067345 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACJSOD_RS09580 (ACJSOD_09580)	pilT	2067560..2068594 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACJSOD_RS09585 (ACJSOD_09585)	pilU	2068772..2069920 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ACJSOD_RS09590 (ACJSOD_09590)	czcD	2069928..2070827 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACJSOD_RS09595 (ACJSOD_09595)	-	2070982..2071386 (+)	405	WP_003084560.1	NINE protein	-
ACJSOD_RS09600 (ACJSOD_09600)	-	2071629..2073002 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
ACJSOD_RS09605 (ACJSOD_09605)	-	2072999..2074183 (+)	1185	WP_124204804.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1077500 ACJSOD_RS09585 WP_003110016.1 2068772..2069920(+) (pilU) [Pseudomonas aeruginosa strain KS1-2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1077500 ACJSOD_RS09585 WP_003110016.1 2068772..2069920(+) (pilU) [Pseudomonas aeruginosa strain KS1-2]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTATATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382