Detailed information NTG1077179

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACJMQR_RS20555
• Genome accession: NZ_CP174465
• Coordinates: 4294531..4295595 (-)
• Length: 354 a.a.
• NCBI ID: WP_136663373.1
• Uniprot ID: A0A4T2A0L6
• Organism: Pseudomonas leptonychotis strain TMP19
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4289531..4300595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJMQR_RS20530 (ACJMQR_20530)	aroK	4290158..4290676 (-)	519	WP_136663369.1	shikimate kinase AroK	-
ACJMQR_RS20535 (ACJMQR_20535)	pilQ	4290681..4292765 (-)	2085	WP_405121185.1	type IV pilus secretin PilQ	Machinery gene
ACJMQR_RS20540 (ACJMQR_20540)	pilP	4292821..4293342 (-)	522	WP_405119175.1	type 4a pilus biogenesis lipoprotein PilP	-
ACJMQR_RS20545 (ACJMQR_20545)	pilO	4293342..4293962 (-)	621	WP_136663371.1	type 4a pilus biogenesis protein PilO	-
ACJMQR_RS20550 (ACJMQR_20550)	-	4293962..4294531 (-)	570	WP_405119176.1	PilN domain-containing protein	-
ACJMQR_RS20555 (ACJMQR_20555)	pilM	4294531..4295595 (-)	1065	WP_136663373.1	pilus assembly protein PilM	Machinery gene
ACJMQR_RS20560 (ACJMQR_20560)	-	4295780..4298194 (+)	2415	WP_405119177.1	penicillin-binding protein 1A	-
ACJMQR_RS20565 (ACJMQR_20565)	-	4298288..4299556 (-)	1269	WP_405119178.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37869.32 Da
• Isoelectric Point: 4.4612

>NTDB_id=1077179 ACJMQR_RS20555 WP_136663373.1 4294531..4295595(-) (pilM) [Pseudomonas leptonychotis strain TMP19]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTVAV
AVAGSAVITKTIEMEAGLSDDELENQLKMEADQYIPYPLEEVAIDFEVQGVSARNADRVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLIDQLGGGRDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVARSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FAEMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1077179 ACJMQR_RS20555 WP_136663373.1 4294531..4295595(-) (pilM) [Pseudomonas leptonychotis strain TMP19]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTACTCGGGATCGATATCAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGCCGCTCCGGAAGCCGCTACAAAGTAGAGGCTTACGCAGTCGAGCCGCTCCCACCAAACGCGGTGGTCGAAAAGAACA
TCGCCGAGTTGGAGGGGGTGGGGCAGGCCCTTTCACGCGTGCTCGCCAAAGCCAAAAGCGGTGTGAAAACCGTTGCGGTG
GCGGTGGCAGGTTCGGCGGTGATTACCAAGACCATCGAGATGGAGGCTGGGCTTTCTGACGATGAGCTGGAAAATCAGCT
GAAGATGGAGGCCGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCTATCGACTTTGAAGTTCAGGGTGTGTCTGCGC
GCAATGCAGATCGTGTCGAAGTGCTGCTTGCCGCTTGCCGCAAAGAAAACGTTGAGGTCCGTGAAGCCGCGTTAGCCCTC
GCGGGTCTTACCGCCAAAGTGGTTGATGTTGAGGCGTACGCACTTGAGCGTGCATATGGCTTGTTGATCGATCAGCTCGG
CGGCGGTCGCGATGAGTTGACCGTTGCGGTTGTTGATATTGGCGCAACCATGACCACCCTCAGTGTTCTGCACAATGGCC
GCACTATTTACACGCGTGAGCAGCTATTCGGCGGCAAACAGCTGACCGAGGAAATCCAGCGACGTTATGGTTTGTCCGTT
GAAGAGGCGGGCCTTGCCAAGAAGCAAGGCGGATTGCCGGATGATTATGACAGTGAAGTACTGCAGCCATTTAAAGAGGC
TGTGGTTCAGCAGGTTGCTCGTTCTCTGCAGTTCTTCTTCGCCGCAGGCCAGTTCAATGATGTTGACTACATCTTGTTGG
CAGGTGGTACCGCGTCAATTCCCGATCTCGATCGTTTGATCCAGCAAAAAATAGGTACCCAGACGCTGGTCGCCAATCCA
TTCGCTGAGATGGCGCTGAGTAGCAAGGTTAACGCCGGCGCGCTCGCCAGTGATGCGCCGGCCTTGATGATTGCTTGTGG
TCTGGCGATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A4T2A0L6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.062	100	0.571
comM	Acinetobacter nosocomialis M2	57.062	100	0.571
comM	Acinetobacter baylyi ADP1	54.237	100	0.542
pilM	Legionella pneumophila strain ERS1305867	46.045	100	0.46