Detailed information NTG1077164

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACJMQR_RS11585
• Genome accession: NZ_CP174465
• Coordinates: 2314571..2315803 (+)
• Length: 410 a.a.
• NCBI ID: WP_405121008.1
• Uniprot ID: -
• Organism: Pseudomonas leptonychotis strain TMP19
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2309571..2320803

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJMQR_RS11565 (ACJMQR_11565)	-	2310051..2311637 (-)	1587	WP_136664701.1	ABC-F family ATPase	-
ACJMQR_RS11570 (ACJMQR_11570)	sph	2312124..2313638 (-)	1515	WP_405121007.1	sphingomyelin phosphodiesterase	-
ACJMQR_RS11575 (ACJMQR_11575)	-	2313772..2314140 (-)	369	WP_405121128.1	MmcQ/YjbR family DNA-binding protein	-
ACJMQR_RS11580 (ACJMQR_11580)	-	2314149..2314427 (-)	279	WP_090253882.1	peptidylprolyl isomerase	-
ACJMQR_RS11585 (ACJMQR_11585)	pilU	2314571..2315803 (+)	1233	WP_405121008.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACJMQR_RS11590 (ACJMQR_11590)	-	2315810..2317765 (-)	1956	WP_405121009.1	acetoacetate--CoA ligase	-
ACJMQR_RS11595 (ACJMQR_11595)	-	2317993..2318895 (-)	903	WP_405121010.1	hydroxymethylglutaryl-CoA lyase	-

Sequence

Protein

• Length: 410 a.a.
• Molecular weight: 44906.24 Da
• Isoelectric Point: 5.5504

>NTDB_id=1077164 ACJMQR_RS11585 WP_405121008.1 2314571..2315803(+) (pilU) [Pseudomonas leptonychotis strain TMP19]
MDLNAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSAEPLKAGEVADIADAVMDVAQREEFERELEMNLAISIAGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLKLPQVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHKHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTIDGKRRAAVEVLLGTPTIRDLIKRNEFSEIKEIMEKSKNLGMQTFDQAL
IDLVNDGSIDEDEAVKNADSANNVRLKLKLYRDNPAAPVQAAAASAPAPAAAEARSTTPAASAGEWGLELTLEDIEEEPL
PPEDPGRKGI

Nucleotide

• Length: 1233 bp

>NTDB_id=1077164 ACJMQR_RS11585 WP_405121008.1 2314571..2315803(+) (pilU) [Pseudomonas leptonychotis strain TMP19]
ATGGATCTCAACGCCATGCTGAAAATCCTGTCCAGCCAGGACGGGTCTGATCTTTACCTTTCGACTGGAGCACCGCCGTG
CGCCAAGTTCAATGGCGTGCTCAAGGCGTTGAGTGCTGAACCGCTGAAAGCCGGCGAGGTGGCGGATATTGCAGACGCGG
TGATGGATGTGGCGCAGCGTGAAGAATTCGAGCGCGAGTTAGAGATGAACCTGGCTATTTCCATTGCTGGCGTCGGGCGC
TTTCGTATCAATATCTTCAAGCAGCGCAACGAAGTGTCGATTGTGGCGCGCAATATCAAGCTGGACATCCCCAAGTTTGA
AGACCTTAAACTTCCGCAGGTGCTGCTCAAGACGGTGATGGAAAAGCGCGGCCTGGTGCTGTTTGTTGGCGGCACGGGCT
CGGGTAAATCGACCTCGCTGGCCGCGTTGATTGATTACCGTAACCGCAACAGCGGTGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATGTACACAAGCACAAAAAGTCGATCATCAACCAACGCGAAGTCGGCGTAGATACCCGCAGTTTTCACGC
GGCACTGAAGAACACCCTGCGCCAGGCCCCAGACGTGATCCTGATCGGCGAAATTCGCGACCGCGAAACCATGGAACATG
CCCTGGCCTTTGCCGACACCGGCCACCTGGCAATTTCCACCCTACACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGACCGCCGCCCGCAACTGCTTAACGACCTGGGTAACAACCTCAAAGCATTCGTCTCGCAGCG
CCTGGTGAAAACCATCGACGGTAAACGCCGCGCGGCGGTTGAAGTACTGCTGGGCACGCCGACCATTCGCGACCTGATCA
AACGCAACGAGTTTTCCGAGATCAAGGAGATCATGGAGAAATCGAAGAACCTCGGCATGCAGACATTCGACCAGGCACTG
ATTGACCTGGTCAACGATGGCTCGATTGACGAAGACGAAGCGGTGAAAAATGCCGACTCGGCAAACAACGTGCGATTGAA
GCTCAAACTGTATCGCGACAATCCAGCTGCGCCGGTGCAGGCCGCTGCAGCCTCGGCACCCGCTCCAGCGGCTGCCGAGG
CACGGTCGACAACCCCAGCCGCTTCAGCGGGCGAGTGGGGGCTGGAGCTGACGCTGGAGGATATCGAAGAGGAGCCACTG
CCGCCTGAAGACCCAGGGCGCAAAGGCATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.41	85.61	0.483
pilU	Acinetobacter baylyi ADP1	52.557	85.854	0.451
pilU	Vibrio cholerae strain A1552	52.125	86.098	0.449
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.734	96.341	0.373